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- bs-5676R-PerCP-Cy5.5
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100ul
| 产品编号 | bs-5676R-PerCP-Cy5.5 |
| 英文名称 | phospho-ROCK2 (Tyr256) Rabbit pAb, PerCP-Cy5.5 conjugated |
| 中文名称 | PerCP-Cy5.5标记的磷酸化Rho相关蛋白激酶2抗体 |
| 英文别名 | ROCK2(phospho Y256); p164 ROCK 2; Rho associated coiled coil containing protein kinase 2; Rho associated protein kinase 2; Rho kinase 2; ROCK 2; Rock II; Rock2m; ROK alpha; ROKalpha; ROCK2_HUMAN. |
| 产品应用 | Flow-Cyt=0.2ug/test, IF=1:100-500 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated Synthesised phosphopeptide derived from human ROCK2 around the phosphorylation site of Tyr256 |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA and 0.02% Proclin300. |
| 研究领域 | Cancer > Signal transduction > Protein phosphorylation > Serine/threonine kinases Cell Biology > Apoptosis > Intracellular > Kinases Signal Transduction > Protein Phosphorylation > Ser / Thr Kinases > Other Kinases |
| 亚基 | Homodimer. Interacts with IRS1, RHOB and RHOC. Interacts with RHOA (activated by GTP), PPP1R12A, CHORDC1, SORL1, EP300 and BRCA2. Interacts with NPM1 and this interaction enhances its activity. Interacts with RAF1. |
| 亚细胞定位 | Cytoplasm. Cell membrane. Cytoplasmic, and associated with actin microfilaments and the plasma membrane. |
| 翻译后修饰 | Phosphorylated upon DNA damage, probably by ATM or ATR.Phosphorylation at Tyr-722 reduces its binding to RHOA and iscrucial for focal adhesion dynamics. Dephosphorylation by PTPN11stimulates its RHOA binding activity. |
| 相似性 | Belongs to the protein kinase superfamily. AGC Ser/Thrprotein kinase family._x000D_
Contains 1 AGC-kinase C-terminal domain._x000D_ Contains 1 PH domain. |
| 功能 | Regulates the assembly of the actin cytoskeleton. Promotes formation of stress fibers and of focal adhesion complexes. Plays a role in smooth muscle contraction. |
| 保存条件 | Store at 2-8°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq, Jul 2008]. |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {0.2ug/test} |
| {IF} | {1:100-500} |
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文献和实验v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
. Immunoblot using antibodies to phospho-Tyr279/216 can assess alterations in activity independent ofthe N-terminal phosphorylation, although phosphorylation atthis site is most likely to be constitutive (14) . 3. Immunoblot using antibodies to known
secondary antibody were purchased from GIBCO-BRL. Phosphospecific rabbit polyclonal antibodies against 180Thr and 182Tyr dual-phosphorylated p38, against total p38 (phosphorylation state independent), against 204Tyr-phosphorylated ERK1/2 and against total
Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array‐Based Functional Protein Studies
.g., Sigma) 50 mg/ml bis‐sulfosuccinimdylsuberate (BS3 ;Pierce, cat. no. PI 21580) in dimethylsulfoxide (DMSO) 5 mg/ml
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