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AFP Rabbit pAb, HRP conjugated

(bs-4821R-HRP)-100ul
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  • ￥2980
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  • bs-4821R-HRP
  • 2025年09月30日
  • 产品信息以Bioss网站为准
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北京博奥森生物技术有限公司
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      100ul

    产品编号bs-4821R-HRP
    英文名称AFP Rabbit pAb, HRP conjugated
    中文名称HRP标记的甲胎蛋白AFP抗体
    英文别名alpha Fetoprotein; alpha-fetoprotein; alpha 1 fetoprotein; alpha-1-fetoprotein; alpha-fetoglobulin; alpha fetoglobulin; alpha Fetoprotein; alpha fetoprotein precursor; FETA; HPAFP; FETA_HUMAN.
    产品应用WB=1:500-2000

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human AFP
    亚型IgG
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Cancer > Tumor biomarkers > Tumor antigens

    Cardiovascular > Blood > Serum Proteins

    Developmental Biology > Organogenesis > Gut development > Liver development

    Kits/ Lysates/ Other > Kits > ELISA Kits > ELISA Kits > Cardiovascular ELISA kits

    亚基Dimeric and trimeric forms have been found in addition to the monomeric form.
    亚细胞定位Secreted.
    组织特异性Plasma. Synthesized by the fetal liver and yolk sac.
    翻译后修饰Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
    Sulfated.
    相似性Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
    功能Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
    保存条件Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料Alpha 1 Fetoprotein is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. Expression has been documented in human adrenal, liver, ovary, testis, and pancreas. ESTs have been isolated from normal human brain, liver/spleen, embryo and uterus tissue libraries.

     

    应用推荐稀释比例
    {WB}{1:500-2000}

     

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    图标文献和实验
    该产品被引用文献

    [IF={{ 5.833 }}] {Chellachamy Anbalagan, Amarnath. et al. Carboxylic acid–tethered polyaniline as a generic immobilization matrix for electrochemical bioassays. Microchim Acta. 2021 Nov;188(11):1-12} {Other} {}

    相关实验

    • 【交流】谁用过RD公司的HRP酶标羊抗鼠IgG试剂盒.

      affinity chromatography and conjugated with horseradish peroxidase (HRP). Formulation Supplied as a 0.2 μm filtered solution in phosphate-buffered saline (PBS) (pH 7.0) with stabilizers. Storage Neat samples can be stored at 2° - 8° C

    • 一种新型酶促化学发光增强液的发光性能及其在免疫分析中的应用

      【摘要】在辣根过氧化物酶/鲁米诺/增强剂/过氧化氢(HRP/luminol/enhancer/H2O2 )增强化学发光体系中，加入一系列辅助试剂，获得了一种灵敏度高、发光持续稳定、可长期贮存的新型酶促化学发光增强液。该增强液灵敏度为 2.4 amol/孔，发光半衰期为 40 min，工作液在室温可稳定存放 1 个月，在37 ℃下可稳定存放14 d。在此基础上，在TSH-BAS ECL EIA和 AFP ECL EIA上进行了应用。TSH-BAS ECL EIA测量范围为 0.17～35 mIU

    • Western Immunoblotting of Proteins

      . For a mouse monoclonal antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes

    查看更多相关实验 >
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    文献支持
    AFP Rabbit pAb, HRP conjugated(bs-4821R-HRP)-100ul
    ￥2980