Blank control:Hela.
Primary Antibody (green line): Mouse Anti-Ki-67 antibody (bsm-33070M-FITC)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Mouse IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
- ￥2980
- Bioss
- bsm-33070M-FITC
- 2025年10月15日
- 产品信息以Bioss网站为准
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
产品信息以Bioss网站为准
- 规格：
100ul
| 产品编号 | bsm-33070M-FITC |
| 英文名称 | Ki-67 Mouse mAb, FITC conjugated |
| 中文名称 | FITC标记的Ki67单克隆抗体 |
| 英文别名 | Antigen identified by monoclonal antibody Ki 67; Antigen KI67; KIA; Ki67; MKI67; Proliferation related Ki 67 antigen; Antigen KI-67; KI67_HUMAN. |
| 产品应用 | Flow-Cyt=1ug/Test Not yet tested in other applications. |
| 抗体来源 | Mouse |
| 免疫原 | KLH conjugated synthetic peptide derived from human Ki-67 |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Monoclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cell cycle > Cell division Cancer > Tumor biomarkers Cell Biology > Cell Cycle > Markers Neuroscience > Cell Type Marker > Neuron marker > Soma marker Neuroscience > Neurology process > Neurogenesis Tags & Cell Markers > Cell Type Markers > Replication |
| 亚细胞定位 | Nucleus. Chromosome. Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes. |
| 相似性 | Contains 1 FHA domain. |
| 功能 | Thought to be required for maintaining cell proliferation. |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation. |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {1ug/Test} |
Blank control:U251.
Primary Antibody (green line): Mouse Anti- antibody (bsm-33070M-FITC)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Mouse IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
Primary Antibody (green line): Mouse Anti- antibody (bsm-33070M-FITC)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Mouse IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
, A., Gertsenstein, M., Vintersten, K., and Behringer, R. 2006a. Preparing mouse embryo fibroblasts. Cold Spring Harb. Protoc. doi: 10.1101/pdb.prot4398.[Free Full Text] Nagy, A., Gertsenstein, M., Vintersten, K., and Behringer, R. 2006b. De novo isolation
Fluorochrome Absorption and Emission Spectra
can be affected by the mAb conjugated. Thus while in the example above the PE-conjugate is brighter than the BD Cy-Chrome™ -conjugate, when analyzed on the BD FACSCalibur™ , for many mAbs the Cy-Chrome ™ -conjugate results in a brighter stain. Contact BD
Fluorochrome Absorption and Emission
can be affected by the mAb conjugated. Thus while in the example above the PE-conjugate is brighter than the BD Cy-Chrome™ -conjugate, when analyzed on the BD FACSCalibur™ , for many mAbs the Cy-Chrome ™ -conjugate results in a brighter stain. Contact BD
技术资料暂无技术资料 索取技术资料
Ki-67 Mouse mAb, FITC conjugated(bsm-33070M-FITC)-100ul
￥2980
