Cleaved PARP1 p25 Recombinant Rabbit mAb(bsm-60941R)-25ul/50ul/100ul

Cleaved PARP1 p25 Recombinant

Rabbit mAb(bsm-60941R)-25ul/50ul/100ul
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  • ￥800 - 2500
  • Bioss已认证
  • bsm-60941R
  • 2025年10月01日
  • 产品信息以Bioss网站为准
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    • 应用范围：

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    • 规格：

      25ul/50ul/100ul

    规格：25ul产品价格：￥800.0
    规格：50ul产品价格：￥1400.0
    规格：100ul产品价格：￥2500.0
    产品编号bsm-60941R
    英文名称Cleaved PARP1 p25 Recombinant Rabbit mAb
    中文名称多腺苷二磷酸多聚酶/多聚ADP-核糖聚合酶1重组兔单抗
    英文别名ADP ribosyltransferase(NAD+; poly(ADP ribose) polymerase); ADP ribosyltransferase NAD+; ADPRT 1; ADPRT; ADPRT1; msPARP; NAD(+) ADP ribosyltransferase 1; pADPRT 1; pADPRT1; PARP 1; PARP; Poly(ADP ribose) polymerase 1; poly(ADP ribose) polymerase family, member 1; Poly adenosine diphosphate ADP ribose polymerase; Poly ADP ribose polymerase 1; Poly ADP ribose polymerase family member 1; Poly ADP ribose synthetase 1; poly(ADP ribose) synthetase; poly(ADP ribosyl)transferase; Poly[ADP ribose] synthetase 1; PPOL; sPARP 1; sPARP1; PARP1_HUMAN.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1:50-200, ICC/IF=1:50-200, IP=1:50-100

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human PARP1
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Recombinant
    理论分子量25/114 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Epigenetics and Nuclear Signaling > Chromatin Modifying Enzymes > ADP-ribosylation

    Epigenetics and Nuclear Signaling > DNA / RNA > DNA Damage & Repair > Base Excision Repair

    Epigenetics and Nuclear Signaling > DNA / RNA > DNA Damage & Repair > DNA Damage Response > DNA Damage Recognition

    亚基Component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LRIG3. Homo- and heterodimer with PARP2. Interacts with PARP3, APTX and SRY. The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70. Interacts with TIAM2 and ZNF423 (By similarity). Interacts (when poly-ADP-ribosylated) with CHD1L. Interacts with the DNA polymerase alpha catalytic subunit POLA1; this interaction functions as part of the control of replication fork progression. Interacts with EEF1A1, RNF4 and TXK.
    亚细胞定位Mitochondrion outer membrane; Single-pass membrane protein.
    Nucleus membrane; Single-pass membrane protein.
    Endoplasmic reticulum membrane; Single-pass membrane protein.
    Nucleus.
    翻译后修饰Phosphorylated by PRKDC and TXK. Phosphorylated upon DNA damage, probably by ATM or ATR._x000D_
    Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites._x000D_
    S-nitrosylated, leading to inhibit transcription regulation activity.
    相似性Contains 1 BRCT domain._x000D_
    Contains 1 PARP alpha-helical domain._x000D_
    Contains 1 PARP catalytic domain._x000D_
    Contains 2 PARP-type zinc fingers.
    功能Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008].

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}
    {Flow-Cyt}{1:50-200}
    {ICC/IF}{1:50-200}
    {IP}{1:50-100}

     

    Cleaved PARP1 p25 Recombinant
    P‌‌araformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cleaved PARP1 p25 Monoclonal Antibody, Unconjugated (bsm-60941R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    Cleaved PARP1 p25 Recombinant
    P‌‌araformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cleaved PARP1 p25 Monoclonal Antibody, Unconjugated (bsm-60941R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    Cleaved PARP1 p25 Recombinant
    P‌‌araformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cleaved PARP1 p25 Monoclonal Antibody, Unconjugated (bsm-60941R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    Cleaved PARP1 p25 Recombinant
    P‌‌araformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cleaved PARP1 p25 Monoclonal Antibody, Unconjugated (bsm-60941R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    Cleaved PARP1 p25 Recombinant
    P‌‌araformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cleaved PARP1 p25 Monoclonal Antibody, Unconjugated (bsm-60941R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    Cleaved PARP1 p25 Recombinant
    P‌‌araformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cleaved PARP1 p25 Monoclonal Antibody, Unconjugated (bsm-60941R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    Cleaved PARP1 p25 Recombinant
    Jurkat (H) cells were treated with or without Staurosporine (1µM) for 4 h, 25 μg total protein per lane of cell lysates (see on figure) probed with Cleaved PARP1 p25 monoclonal antibody, unconjugated (bsm-60941R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

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