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- 规格：
25ul/50ul/100ul
| 规格： | 25ul | 产品价格： | ￥800.0 |
|---|---|---|---|
| 规格： | 50ul | 产品价格： | ￥1400.0 |
| 规格： | 100ul | 产品价格： | ￥2500.0 |
| 产品编号 | bsm-63104R |
| 英文名称 | phospho-DNA PKcs (S2056) Recombinant Rabbit mAb |
| 中文名称 | 磷酸化DNA依赖性蛋白激酶重组兔单抗 |
| 英文别名 | DNA- PKcs; DNA-dependent protein kinase catalytic subunit; DNPK1; EC 2.7.11.1; P460; PRKD; PRKDC; XRCC7; kinase DNA-PK. |
| 产品应用 | WB=1:1000-2000, IHC-P=1:100-200, IHC-F=1:100-200, ICC/IF=1:50-200, IF=1:100-200 Not yet tested in other applications. |
| 交叉反应 | Human |
| 抗体来源 | Rabbit |
| 免疫原 | A synthesized peptide derived from human DNA PKcs around the phosphorylation site of S2056 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Recombinant |
| 理论分子量 | 469 kDa |
| 实际分子量 | 469kDa |
| 储存液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > DNA / RNA > DNA Damage & Repair > DNA Damage Response > DNA Damage Recognition Epigenetics and Nuclear Signaling > DNA / RNA > DNA Damage & Repair > Non Homol. End Joining |
| 亚基 | DNA-PK is a heterotrimer of PRKDC and the Ku p70-p86 (XRCC6-XRCC5) dimer. Formation of this complex may be promoted by interaction with ILF3. Associates with the DNA-bound Ku heterodimer, but it can also bind to and be activated by free DNA. Interacts with DNA-PKcs-interacting protein (KIP) with the region upstream the kinase domain. PRKDC alone also interacts with and phosphorylates DCLRE1C, thereby activating the latent endonuclease activity of this protein. Interacts with C1D. Interacts with TTI1 and TELO2. |
| 亚细胞定位 | Nucleus. |
| 翻译后修饰 | Phosphorylated upon DNA damage, probably by ATM or ATR. Autophosphorylated on Thr-2609, Thr-2638 and Thr-2647. Thr-2609 is a DNA damage-inducible phosphorylation site (inducible with ionizing radiation, IR). Autophosphorylation induces a conformational change that leads to remodeling of the DNA-PK complex, requisite for efficient end processing and DNA repair._x000D_
S-nitrosylated by GAPDH. |
| 相似性 | Belongs to the PI3/PI4-kinase family._x000D_
Contains 1 FAT domain._x000D_ Contains 1 FATC domain._x000D_ Contains 2 HEAT repeats._x000D_ Contains 1 PI3K/PI4K domain._x000D_ Contains 3 TPR repeats |
| 功能 | Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:1000-2000} |
| {IHC-P} | {1:100-200} |
| {IHC-F} | {1:100-200} |
| {ICC/IF} | {1:50-200} |
| {IF} | {1:100-200} |
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phospho-DNA PKcs (S2056) Recombinant Rabbit mAb(bsm-63104R)-25ul/50ul/100ul
￥800 - 2500
