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IGF2BP2 Recombinant Rabbit mAb

(bsm-62659R)-50ul/100ul/25ul
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  • ￥800 - 2500
  • Bioss已认证
  • bsm-62659R
  • 2025年10月01日
  • 产品信息以Bioss网站为准
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    • 规格：

      50ul/100ul/25ul

    规格：50ul产品价格：￥1400.0
    规格：100ul产品价格：￥2500.0
    规格：25ul产品价格：￥800.0
    产品编号bsm-62659R
    英文名称IGF2BP2 Recombinant Rabbit mAb
    中文名称胰岛素样生长因子ⅡmRNA结合蛋白2重组兔单抗
    英文别名gf2bp2; IMP2; p62; VICKZ2.
    产品应用WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse, Rat
    抗体来源Rabbit
    免疫原A synthesized peptide derived from human IGF2BP2
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Recombinant
    理论分子量66
    实际分子量66,62
    储存液10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
    研究领域

    Epigenetics and Nuclear Signaling > Chromatin Binding Proteins > DNA / RNA binding

    Epigenetics and Nuclear Signaling > DNA / RNA > Translation > Regulation

    Immunology > Immune System Diseases > Autoimmune

    Metabolism > Types of disease > Metabolic disorders

    Signal Transduction > Growth Factors/Hormones > Insulin / Insulin-like

    亚基Interacts with HNRPD and IGF2BP1.
    亚细胞定位Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Localizes at the connecting piece and the tail of the spermatozoa.
    组织特异性Expressed in oocytes, granulosa cells of small and growing follicles, Leydig cells, spermatogonia and semen (at protein level). Expressed in testicular cancer (at protein level). Expressed weakly in heart, placenta, skeletal muscle, bone marrow, colon, kidney, salivary glands, testis and pancreas. Detected in fetal liver, fetal ovary, gonocytes and interstitial cells of the testis.
    相似性Belongs to the RRM IMP/VICKZ family_x000D_ br>Contains 4 KH domains._x000D_
    Contains 2 RRM (RNA recognition motif) domains.
    功能Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. May regulate translation of target mRNAs.
    保存条件Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料RNA-binding factor that recruits target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation.Preferentially binds to N6-methyladenosine (m6A)-containing mRNAs and increases their stability.

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:50-200}
    {IHC-F}{1:50-200}
    {IF}{1:50-200}

     

    产品细节图片1
    25 ug total protein per lane of various lysates (see on figure) probed with IGF2BP2 monoclonal antibody, unconjugated (bsm-62659R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
    产品细节图片2
    P‌‌araformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IGF2BP2 Monoclonal Antibody, Unconjugated(bsm-62659R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    产品细节图片3
    P‌‌araformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IGF2BP2 Monoclonal Antibody, Unconjugated(bsm-62659R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    产品细节图片4
    P‌‌araformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IGF2BP2 Monoclonal Antibody, Unconjugated(bsm-62659R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    产品细节图片5
    P‌‌araformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IGF2BP2 Monoclonal Antibody, Unconjugated(bsm-62659R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

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