- ￥800 - 2500
- Bioss
- bsm-62636R
- 2025年10月01日
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- 规格：
50ul/100ul/25ul
| 规格： | 50ul | 产品价格： | ￥1400.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2500.0 |
| 规格： | 25ul | 产品价格： | ￥800.0 |
| 产品编号 | bsm-62636R |
| 英文名称 | AKAP95 Recombinant Rabbit mAb |
| 中文名称 | 蛋白激酶A锚定蛋白95重组兔单抗 |
| 英文别名 | Akap8; AKAP95. |
| 产品应用 | WB=1:1000-2000, IHC-P=1:100-200, IHC-F=1:100-200, ICC/IF=1:50-200, IF=1:50-200 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat |
| 抗体来源 | Rabbit |
| 免疫原 | A synthesized peptide derived from human AKAP 8 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Recombinant |
| 理论分子量 | 76 |
| 实际分子量 | 95 |
| 储存液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 研究领域 | Cell Biology > Cell Cycle > Kinases/Phosphatases |
| 亚基 | Interacts with PRPF40A. Binds to the C-terminal of RNA helicase A and to TMPO-beta. Interacts with RNF43. |
| 亚细胞定位 | Nucleus matrix. Cytoplasm. Note=Colocalizes with PRPF40A in the nuclear matrix. Nuclear at steady state but shuttles between the nucleus and cytoplasm. Associated with the nuclear matrix. |
| 组织特异性 | Expressed in the brain cortex (at protein level). |
| 翻译后修饰 | Phosphorylated on serine or threonine residues possibly by PKA. |
| 相似性 | Belongs to the AKAP95 family._x000D_
Contains 2 C2H2 AKAP95-type zinc fingers. |
| 功能 | Could play a role in constitutive transport element (CTE)-mediated gene expression. Does not seem to be implicated in the binding of regulatory subunit II of PKA. May be involved in nuclear envelope breakdown and chromatin condensation. May regulate the initiation phase of DNA replication when associated with TMPO-beta. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II). Acts as an anchor for a PKA-signaling complex onto mitotic chromosomes, which is required for maintenance of chromosomes in a condensed form throughout mitosis. Recruits condensin complex subunit NCAPD2 to chromosomes required for chromatin condensation; the function appears to be independent from PKA-anchoring. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:1000-2000} |
| {IHC-P} | {1:100-200} |
| {IHC-F} | {1:100-200} |
| {ICC/IF} | {1:50-200} |
| {IF} | {1:50-200} |
Western blot analysis of HepG2 cell lysate. Using AKAP95 (bsm-62636R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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AKAP95 Recombinant Rabbit mAb(bsm-62636R)-50ul/100ul/25ul
￥800 - 2500
