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CRMP3 Recombinant Rabbit mAb(b

sm-62461R)-50ul/100ul/25ul
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  • ¥800 - 2500
  • Bioss已认证
  • bsm-62461R
  • 2025年10月01日
  • 产品信息以Bioss网站为准
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    • 规格

      50ul/100ul/25ul

    规格:50ul产品价格:¥1400.0
    规格:100ul产品价格:¥2500.0
    规格:25ul产品价格:¥800.0
    产品编号bsm-62461R
    英文名称CRMP3 Recombinant Rabbit mAb
    中文名称二氢嘧啶酶相关蛋白3重组兔单抗
    英文别名CRMP3; DPYSL4; DRP4; ULIP4.
    产品应用WB=1:1000-2000, Flow-Cyt=1:50-100

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse, Rat
    抗体来源Rabbit
    免疫原A synthesized peptide derived from human DRP 4
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Recombinant
    理论分子量62
    实际分子量62
    储存液10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
    研究领域

    Neuroscience > Neurology process > Neurogenesis

    Neuroscience > Neurology process > Neuroregeneration > Neuroregeneration

    亚细胞定位Cytoplasm.
    组织特异性Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.
    翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
    相似性Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.
    功能Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration.
    保存条件Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration.

     

    应用推荐稀释比例
    {WB}{1:1000-2000}
    {Flow-Cyt}{1:50-100}

     

    产品细节图片1
    Western blot analysis of SH-SY5Y cell lysate. Using CRMP3 (bsm-62461R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
    产品细节图片2
    The SH-SY5Y (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-CRMP3 antibody (bsm-62461R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

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