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- bsm-62448R
- 2025年10月01日
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产品信息以Bioss网站为准
- 规格：
50ul/100ul/25ul
| 规格： | 50ul | 产品价格： | ￥1400.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2500.0 |
| 规格： | 25ul | 产品价格： | ￥800.0 |
| 产品编号 | bsm-62448R |
| 英文名称 | CTBP2 Recombinant Rabbit mAb |
| 中文名称 | C末端结合蛋白2重组兔单抗 |
| 英文别名 | CtBP2; ribeye. |
| 产品应用 | WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, ICC/IF=1:50-200, IF=1:50-200, Flow-Cyt=1:50-100 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat |
| 抗体来源 | Rabbit |
| 免疫原 | A synthesized peptide derived from human CtBP2 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Recombinant |
| 理论分子量 | 49 |
| 实际分子量 | 47 |
| 储存液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > Transcription > Co-factors Epigenetics and Nuclear Signaling > Transcription > Other factors Microbiology > Organism > Virus > DNA Virus > double stranded DNA Virus > Adenovirus Neuroscience > Cell Type Marker > Neuron marker > Synapse marker |
| 亚基 | Interacts with the C-terminus of adenovirus E1A protein. Can form homodimers or heterodimers of CTBP1 and CTBP2. Interacts with HIPK2 and ZNF217. Interacts with PRDM16; represses white adipose tissue (WAT)-specific genes expression. Interacts with PNN, NRIP1 and WIZ. |
| 亚细胞定位 | Nucleus. Cell junction, synapse. |
| 组织特异性 | Ubiquitous. Highest levels in heart, skeletalv muscle, and pancreas. |
| 相似性 | Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. |
| 功能 | Corepressor targeting diverse transcription regulators. Functions in brown adipose tissue (BAT) differentiation._x000D_
Isoform 2 probably acts as a scaffold for specialized synapses. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Corepressor targeting diverse transcription regulators. Functions in brown adipose tissue (BAT) differentiation. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:50-200} |
| {IHC-F} | {1:50-200} |
| {ICC/IF} | {1:50-200} |
| {IF} | {1:50-200} |
| {Flow-Cyt} | {1:50-100} |
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CTBP2 Monoclonal Antibody, Unconjugated (bsm-62448R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CTBP2 Monoclonal Antibody, Unconjugated (bsm-62448R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CTBP2 Monoclonal Antibody, Unconjugated (bsm-62448R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CTBP2 Monoclonal Antibody, Unconjugated (bsm-62448R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CTBP2 Monoclonal Antibody, Unconjugated (bsm-62448R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CTBP2 Monoclonal Antibody, Unconjugated (bsm-62448R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CTBP2 Monoclonal Antibody, Unconjugated (bsm-62448R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CTBP2 Monoclonal Antibody, Unconjugated (bsm-62448R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CTBP2 Monoclonal Antibody, Unconjugated (bsm-62448R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CTBP2 Monoclonal Antibody, Unconjugated (bsm-62448R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CTBP2 Monoclonal Antibody, Unconjugated (bsm-62448R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
25 ug total protein per lane of various lysates (see on figure) probed with CTBP2 monoclonal antibody, unconjugated (bsm-62448R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-CTBP2 antibody (bsm-62448R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (CTBP2) monoclonal Antibody, unconjugated (bsm-62448R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
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CTBP2 Recombinant Rabbit mAb(bsm-62448R)-50ul/100ul/25ul
￥800 - 2500
