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- 详细信息
- 技术资料
- 应用范围:
产品信息以Bioss网站为准
- 规格:
50ul/100ul/25ul
| 规格: | 50ul | 产品价格: | ¥1400.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2500.0 |
| 规格: | 25ul | 产品价格: | ¥800.0 |
| 产品编号 | bsm-62442R |
| 英文名称 | THEMIS Recombinant Rabbit mAb |
| 中文名称 | 选择性胸腺细胞相关蛋白重组兔单抗 |
| 英文别名 | Protein THEMIS; SPOT; THEMIS; TSEPA. |
| 产品应用 | WB=1:500-2000, ICC/IF=1:50-200, IP=1:20-50, Flow-Cyt=1:50-100 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat |
| 抗体来源 | Rabbit |
| 免疫原 | A synthesized peptide derived from human THEMIS |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Recombinant |
| 理论分子量 | 73 |
| 实际分子量 | 73 |
| 储存液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 研究领域 | Immunology > Adaptive Immunity > T Cells > Non-CD Stem Cells > Hematopoietic Progenitors > Lymphoid > T Lymphocytic Lineage |
| 亚细胞定位 | Cytoplasm. Nucleus. |
| 翻译后修饰 | Phosphorylated on Tyr residues quickly after TCR stimulation. |
| 相似性 | Belongs to the themis family. |
| 功能 | Plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells. Regulates T-cell development through T-cell antigen receptor (TCR) signaling and in particular through the regulation of calcium influx and phosphorylation of Erk. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells. Regulates T-cell development through T-cell antigen receptor (TCR) signaling and in particular through the regulation of calcium influx and phosphorylation of Erk. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {ICC/IF} | {1:50-200} |
| {IP} | {1:20-50} |
| {Flow-Cyt} | {1:50-100} |

Western blot analysis of Jurkat cell lysate. Using THEMIS (bsm-62442R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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THEMIS Recombinant Rabbit mAb(bsm-62442R)-50ul/100ul/25ul
¥800 - 2500





