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- 技术资料
- 应用范围:
产品信息以Bioss网站为准
- 规格:
50ul/100ul/25ul
| 规格: | 50ul | 产品价格: | ¥1400.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2500.0 |
| 规格: | 25ul | 产品价格: | ¥800.0 |
| 产品编号 | bsm-62224R |
| 英文名称 | MYF5 Recombinant Rabbit mAb |
| 中文名称 | 生肌决定因子Myf5重组兔单抗 |
| 英文别名 | Myf5; Class C basic helix loop helix protein 2. |
| 产品应用 | WB=1:500-2000, IP=1:20-50 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human) |
| 抗体来源 | Rabbit |
| 免疫原 | A synthesized peptide derived from human MYF5 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Recombinant |
| 理论分子量 | 28 |
| 实际分子量 | 37 |
| 储存液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 研究领域 | Developmental Biology > Organogenesis > Skeletal development > Muscle Epigenetics and Nuclear Signaling > Transcription > Domain Families > HLH / Leucine Zipper > Helix-Turn-Helix Epigenetics and Nuclear Signaling > Transcription > Transcription Factors Stem Cells > Mesenchymal Stem Cells > Myogenesis |
| 亚基 | Efficient DNA binding requires dimerization with another bHLH protein. |
| 亚细胞定位 | Nucleus. |
| 相似性 | Contains 1 bHLH (basic helix-loop-helix) domain. |
| 功能 | Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYOG and MYOD1, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IP} | {1:20-50} |

25 ug total protein per lane of various lysates (see on figure) probed with MYF5 monoclonal antibody, unconjugated (bsm-62224R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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MYF5 Recombinant Rabbit mAb(bsm-62224R)-50ul/100ul/25ul
¥800 - 2500





