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- 详细信息
- 技术资料
- 应用范围:
产品信息以Bioss网站为准
- 规格:
50ul/100ul/25ul
| 规格: | 50ul | 产品价格: | ¥1400.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2500.0 |
| 规格: | 25ul | 产品价格: | ¥800.0 |
| 产品编号 | bsm-61894R |
| 英文名称 | USP9X Recombinant Rabbit mAb |
| 中文名称 | 泛素特异性蛋白酶9X重组兔单抗 |
| 英文别名 | DFFRX; FAF; Fafl; Fam; hFAM; MRX99; Usp9x; X chromosome; X-linked. |
| 产品应用 | WB=1:1000-2000, IHC-P=1:100-200, IHC-F=1:100-200, ICC/IF=1:50-200, IF=1:50-200, Flow-Cyt=1:50-100 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat |
| 抗体来源 | Rabbit |
| 免疫原 | A synthesized peptide derived from human USP9X |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Recombinant |
| 理论分子量 | 290 |
| 实际分子量 | 292 |
| 储存液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 研究领域 | Cell Biology > Proteolysis / Ubiquitin > Proteasome / Ubiquitin > Deubiquitination Cell Biology > Proteolysis / Ubiquitin > Proteasome / Ubiquitin > Ub-like Proteins Epigenetics and Nuclear Signaling > Ubiquitin & Ubiquitin Like Modifiers > Deubiquitination Neuroscience > Neurology process > Neurogenesis |
| 亚基 | Interacts with SMAD4, MARK4, NUAK1 and BIRC5/survivin. |
| 亚细胞定位 | Cytoplasm. |
| 组织特异性 | Widely expressed in embryonic and adult tissues. |
| 相似性 | Belongs to the peptidase C19 family. |
| 功能 | Deubiquitinase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. May therefore play an important role regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin. Essential component of TGF-beta/BMP signaling cascade. Regulates chromosome alignment and segregation in mitosis by regulating the localization of BIRC5/survivin to mitotic centromeres. Specifically hydrolyzes both 'Lys-29'- and 'Lys-33'-linked polyubiquitins chains. Specifically deubiquitinates monoubiquitinated SMAD4, opposing the activity of E3 ubiquitin-protein ligase TRIM33. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Deubiquitinase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins.May therefore play an important regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:1000-2000} |
| {IHC-P} | {1:100-200} |
| {IHC-F} | {1:100-200} |
| {ICC/IF} | {1:50-200} |
| {IF} | {1:50-200} |
| {Flow-Cyt} | {1:50-100} |

Western blot analysis of HeLa cell lysate. Using USP9X (bsm-61894R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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USP9X Recombinant Rabbit mAb(bsm-61894R)-50ul/100ul/25ul
¥800 - 2500





