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- bsm-61876R
- 2025年10月01日
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产品信息以Bioss网站为准
- 规格：
50ul/100ul/25ul
| 规格： | 50ul | 产品价格： | ￥1400.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2500.0 |
| 规格： | 25ul | 产品价格： | ￥800.0 |
| 产品编号 | bsm-61876R |
| 英文名称 | GRASP65 Recombinant Rabbit mAb |
| 中文名称 | 高尔基体外周膜蛋白p65重组兔单抗 |
| 英文别名 | GOLPH5; GORASP1; GRASP65. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-200, IHC-F=1:100-200, ICC/IF=1:50-200, IF=1:100-200, IP=1:20-50, Flow-Cyt=1μg/Test Not yet tested in other applications. |
| 交叉反应 | Human |
| 抗体来源 | Rabbit |
| 免疫原 | A synthesized peptide derived from human GORASP1 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Recombinant |
| 理论分子量 | 46 |
| 实际分子量 | 65 |
| 储存液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 研究领域 | Cell Biology > Cell Cycle > Cell Division > Other cell division antibodies Tags & Cell Markers > Subcellular Markers > Organelles > Golgi |
| 亚基 | Homodimer. Forms higher order oligomers under interphase but not mitotic conditions. Dimers of the protein on one membrane might be able to interact with dimers on another and so stack cisternae. Interacts with the C-terminus of GM130 under both mitotic and non-mitotic conditions. The interaction is critical for the correct targeting of both proteins to the cis-Golgi. The complex binds to the vesicle docking protein p115. Interacts with TMED2 and TMED3 (By similarity). |
| 亚细胞定位 | Golgi apparatus, cis-Golgi network membrane; |
| 翻译后修饰 | Phosphorylated by CDC2/B1 and PLK kinases during mitosis. Phosphorylation cycle correlates with the cisternal stacking cycle. Phosphorylation of the homodimer prevents the association of dimmers into higher order oligomers, leading to cisternal unstacking. Target for caspase-3 cleavage during apoptosis. The cleavage contributes to Golgi fragmentation and occurs very early in the execution phase of apoptosis (By similarity). |
| 相似性 | Belongs to the GORASP family. Contains 1 PDZ (DHR) domain. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Key structural protein of the Golgi apparatus.The membrane cisternae of the Golgi apparatus adhere to each other to form stacks, which are aligned side by side to form the Golgi ribbon.Acting in concert with GORASP2/GRASP55, is required for the formation and maintenance of the Golgi ribbon, and may be dispensable for the formation of stacks. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-200} |
| {IHC-F} | {1:100-200} |
| {ICC/IF} | {1:50-200} |
| {IF} | {1:100-200} |
| {IP} | {1:20-50} |
| {Flow-Cyt} | {1μg/Test} |
25 ug total protein per lane of various lysates (see on figure) probed with GRASP65 monoclonal antibody, unconjugated (bsm-61876R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (GRASP65) monoclonal Antibody, unconjugated (bsm-61876R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
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GRASP65 Recombinant Rabbit mAb(bsm-61876R)-50ul/100ul/25ul
￥800 - 2500
