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50ul/100ul/25ul
| 规格： | 50ul | 产品价格： | ￥1400.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2500.0 |
| 规格： | 25ul | 产品价格： | ￥800.0 |
| 产品编号 | bsm-61334R |
| 英文名称 | CDC45L Recombinant Rabbit mAb |
| 中文名称 | 细胞分裂周期调控蛋白45重组兔单抗 |
| 英文别名 | CDC45L; CDC45L2; PORC-PI-1; CDC45. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-200, IHC-F=1:100-200, ICC/IF=1:50-200, IF=1:50-200, Flow-Cyt=1:50-100 Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat) |
| 抗体来源 | Rabbit |
| 免疫原 | A synthesized peptide derived from human CDC45 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Recombinant |
| 理论分子量 | 66 |
| 实际分子量 | 65 |
| 储存液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 研究领域 | Cell Biology > Cell Cycle > Cell Division > Other cell division antibodies Epigenetics and Nuclear Signaling > DNA / RNA > DNA Synthesis Epigenetics and Nuclear Signaling > DNA / RNA > DNA Synthesis > DNA Polymerases |
| 亚基 | Associated with ORC2. |
| 亚细胞定位 | Cytoplasm. Nucleus. |
| 组织特异性 | Widely expressed, highest levels are found in adult testis and thymus and in fetal liver. |
| 相似性 | Belongs to the CDC45 family. |
| 功能 | Required for initiation of chromosomal DNA replication.< |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Required for initiation of chromosomal DNA replication. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-200} |
| {IHC-F} | {1:100-200} |
| {ICC/IF} | {1:50-200} |
| {IF} | {1:50-200} |
| {Flow-Cyt} | {1:50-100} |
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文献和实验[IF={{ 5 }}] {Qi Jiang. et al. Itaconate Ameliorates Experimental Autoimmune Uveitis by Modulating Teff/Treg Cell Imbalance Via the DNAJA1/CDC45 Axis. INVEST OPHTH VIS SCI. 2024 Dec;65(14):23-23} {FC} {Mouse}
到 PVDF 膜上，同时减少蛋白不必要降解，这对于最终获得清晰、可信结果也是需要考虑因素。抗体信息：1.ACC1, Recombinant Rabbit monoclonal IgG. HuaAn. HuaAn biotechnology , inc.2.ATGL, Mouse mAb IgG1, Cat#:RT1058. HuaAn biotechnology , inc.3.p-PERK(Thr981), Rabbit Polyclonal IgG primary antibodies, Cat
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
al. 2006b ). MATERIALS Reagents Anti-CD24 monoclonal antibody (mAb) (J11d clone) (for Protocol 3) Use either culture supernatant that contains anti-CD24 mAb or purified anti-CD24 mAb (see Step 55). BDPharmLyse (red blood cell lysing
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