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- bsm-33175M-HRP
- 2025年09月30日
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100ul
| 产品编号 | bsm-33175M-HRP |
| 英文名称 | mStrawberry Mouse mAb, HRP conjugated |
| 中文名称 | HRP标记的单体橙红色荧光蛋白单克隆抗体 |
| 英文别名 | monomeric red-orange fluorescent protein; |
| 产品应用 | WB=1:1000-5000 Not yet tested in other applications. |
| 抗体来源 | Mouse |
| 免疫原 | Recombinant Protein mStrawberry |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Monoclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:1000-5000} |
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文献和实验我是用来做筛选IgG型单克隆抗体,用它做二抗,筛到的阳性克隆化和扩大培养后用SIGMA亚类试剂盒鉴定却是IgM,郁闷!!!这个试剂盒能与IGM交叉?是不是抗轻链?我这种方式不对吗?谁知道有较好的抗鼠IgG而与IgM不交叉的抗体?? 试剂盒说明书:Anti-mouse IgG-HRP Antibody FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. R&D Systems, Inc. 1-800-343-7475 2/21/06
. For alkaline phosphatase, use a 1:3000 dilution of Biorad goat anti-mouse or anti-rabbit alkaline phosphatase conjugated antibody in blotto. Incubate on rocker 1 hr at 25℃. 8. Wash 3X3min in TBS/Tween. 9. Alkaline phosphatase development: Add developing
for 6 months without reducing binding activity. 2) This buffer can not be used for diluting HRP conjugated antibodies since sodium azide is an inhibitor of HRP. HRP-conjugated Primary Antibody Dilution Buffer: 1%BSA
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