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- Bioss
- bs-7540R-Bio
- 2025年09月30日
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100ul
| 产品编号 | bs-7540R-Bio |
| 英文名称 | GRK5 Rabbit pAb, Biotin conjugated |
| 中文名称 | Biotin标记的G蛋白偶联受体激酶5抗体 |
| 英文别名 | G protein coupled receptor kinase 5; G protein coupled receptor kinase GRK5; G protein-coupled receptor kinase 5; G protein-coupled receptor kinase GRK5; GPRK5; GRK5; GRK5_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human GRK5 |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cardiovascular > Atherosclerosis > Hypertension > Vascular remodelling > VSMC migration Cardiovascular > Atherosclerosis > Vascular Inflammation > Leukocyte recruitment Cardiovascular > Blood > Blood Pressure regulation Signal Transduction > Protein Phosphorylation > Ser / Thr Kinases > GPCR Kinases Signal Transduction > Signaling Pathway > G Protein Signaling > GPCR Signal Transduction > Signaling Pathway > G Protein Signaling > Heterotrimeric G Proteins > GPCR Kinases |
| 亚基 | Interacts with ST13 (via the C-terminus 303-319 AA). Interacts with TP53/p53. Interacts with HTR4 (via C-terminus 330-346 AA); this interaction is promoted by 5-HT (serotonin). Interacts with HDAC5. |
| 亚细胞定位 | Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein. Note=Predominantly localized at the plasma membrane; targeted to the cell surface through the interaction with phospholipids. Nucleus localization is regulated in a GPCR and Ca(2+)/calmodulin-dependent fashion. |
| 组织特异性 | Highest levels in heart, placenta, lung, skeletal muscle, brain, liver, pancreas, kidney. |
| 翻译后修饰 | Autophosphorylated. Autophosphorylation may play a critical role in the regulation of GRK5 kinase activity. |
| 相似性 | Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily._x000D_
Contains 1 AGC-kinase C-terminal domain._x000D_ Contains 1 protein kinase domain._x000D_ Contains 1 RGS domain. |
| 功能 | Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein-coupled receptors (GPCRs). Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation. Phosphorylates a variety of GPCRs, including adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4 subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, also phosphorylates various substrates: Hsc70-interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1. Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. Phosphorylation of HDAC5, a repressor of myocyte enhancer factor 2 (MEF2) leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription. Phosphorylation of TP53/p53, a crucial tumor suppressor, inhibits TP53/p53-mediated apoptosis. Phosphorylation of ST13 regulates internalization of the chemokine receptor. Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor, LRP6 during Wnt signaling (in vitro). |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. It has also been shown to play a role in regulating the motility of polymorphonuclear leukocytes (PMNs). [provided by RefSeq, Jul 2008]. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
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GRK5 Rabbit pAb, Biotin conjugated(bs-7540R-Bio)-100ul
￥2980
