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- Bioss
- bs-7862R-HRP
- 2025年09月30日
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100ul
| 产品编号 | bs-7862R-HRP |
| 英文名称 | FAM3C Rabbit pAb, HRP conjugated |
| 中文名称 | HRP标记的上皮分泌型FAM3C蛋白抗体 |
| 英文别名 | Fam3c; FAM3C_HUMAN; family with sequence similarity 3, member C; GS3786; ILEI; Interleukin-like EMT inducer; Predicted osteoblast protein; Protein FAM3C. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human FAM3C |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cell Biology > Cell Cycle > Cell differentiation Immunology > Secreted Molecules > Other secreted molecules |
| 亚细胞定位 | Secreted. Cytoplasmic vesicle. Note=Cytoplasmic in some cancer cells. |
| 组织特异性 | Present in most secretory epithelia (at protein level). |
| 相似性 | Belongs to the FAM3 family. |
| 功能 | May be involved in retinal laminar formation. Promotes epithelial to mesenchymal transition. |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | ILEI is a 227 amino acid, ubiquitously expressed protein containing an amino-terminal signal peptide. Elevated levels of ILEI translation are observed in oncogenic, Ras-transformed mammary epithelial cells and epithelial to mesenchymal transition (Emt) as well as tumor growth and metastasis. Also, overexpression of ILEI results in loss of ZO-1, a protein involved in tight junctions, and expression of cytoplasmic E-cadherin, which has been shown to influence loss of polarity and invasiveness. Due to this evidence, it is suspected that ILEI cooperates with oncogenic Ras to cause TGF∫-independent Emt and its overexpression is correlated with the invasion, metastasis and survival in a variety of cancers. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
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文献和实验affinity chromatography and conjugated with horseradish peroxidase (HRP). Formulation Supplied as a 0.2 μm filtered solution in phosphate-buffered saline (PBS) (pH 7.0) with stabilizers. Storage Neat samples can be stored at 2° - 8° C
Western Immunoblotting of Proteins
. For a mouse monoclonal antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes
Western Immunoblotting of Proteins
antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes each with either water
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