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- bs-16050R-HRP
- 2025年09月30日
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100ul
| 产品编号 | bs-16050R-HRP |
| 英文名称 | FBXO44 Rabbit pAb, HRP conjugated |
| 中文名称 | HRP标记的FBXO44蛋白抗体 |
| 英文别名 | 5730411K09; AV001623; DKFZp781J0852; F box gene 3; F box only protein 44; F box only protein 6a; F box protein 44; F box protein FBX30; F box/G domain protein 3; F-box only protein 44; F-box protein FBX30; F-box/G-domain protein 3; FBG3; FBX30; FBX44; FBX44_HUMAN; FBX6A; Fbxo44; Fbxo6a; MGC14140; RGD1562463. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human FBXO44 |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cell Biology > Proteolysis / Ubiquitin > Proteasome / Ubiquitin > Ubiquitin E3 Enzymes > F Box E3 Ligase |
| 亚基 | Part of a SCF (SKP1-cullin-F-box) protein ligase complex. Interacts with SKP1 and CUL1._x000D_ |
| 组织特异性 | Abundantly expressed in brain and kidney. Expressed at lower levels in heart, spleen and liver. |
| 相似性 | Contains 1 F-box domain. _x000D_ Contains 1 FBA (F-box associated) domain. |
| 功能 | Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. It is also a member of the NFB42 (neural F Box 42 kDa) family, similar to F-box only protein 2 and F-box only protein 6. Four alternatively spliced transcript variants encoding two distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
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文献和实验affinity chromatography and conjugated with horseradish peroxidase (HRP). Formulation Supplied as a 0.2 μm filtered solution in phosphate-buffered saline (PBS) (pH 7.0) with stabilizers. Storage Neat samples can be stored at 2° - 8° C
Western Immunoblotting of Proteins
. For a mouse monoclonal antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes
Western Immunoblotting of Proteins
antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes each with either water
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