Blank control（black line）:A549.
Primary Antibody (green line): Rabbit Anti-SFTPC antibody (bs-10067R)-PE
Dilution:1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Negative control（white blue line）: PBS
Isotype control（orange line）: Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
- ￥2980
- Bioss
- bs-10067R-PE
- 2025年10月15日
- 产品信息以Bioss网站为准
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产品信息以Bioss网站为准
- 规格：
100ul
| 产品编号 | bs-10067R-PE |
| 英文名称 | SFTPC Rabbit pAb, PE conjugated |
| 中文名称 | PE标记的肺表面活性蛋白C抗体 |
| 英文别名 | PSP C; PSPC; Pulmonary surfactant apoprotein 2; pulmonary surfactant apoprotein PSP C; pulmonary surfactant associated protein C; pulmonary surfactant associated proteolipid SPL pVal; Pulmonary surfactant associated proteolipid SPL(Val); SFTP 2; SFTP2; SP-C; SFTPC surfactant pulmonary associated protein C; SP 5; SP C; SP5; SPC; surfactant associated protein pulmonary 2; Surfactant protein c; Surfactant pulmonary associated protein C; PSPC_HUMAN. |
| 产品应用 | Flow-Cyt=1μg/Test Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human SFTPC |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚细胞定位 | Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. |
| 相似性 | Contains 1 BRICHOS domain. |
| 功能 | Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes the pulmonary-associated surfactant protein C (SPC), an extremely hydrophobic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a surface-active lipoprotein complex composed of 90% lipids and 10% proteins which include plasma proteins and apolipoproteins SPA, SPB, SPC and SPD. The surfactant is secreted by the alveolar cells of the lung and maintains the stability of pulmonary tissue by reducing the surface tension of fluids that coat the lung. Multiple mutations in this gene have been identified, which cause pulmonary surfactant metabolism dysfunction type 2, also called pulmonary alveolar proteinosis due to surfactant protein C deficiency, and are associated with interstitial lung disease in older infants, children, and adults. Alternatively spliced transcript variants encoding different protein isoforms have been identified. |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {1μg/Test} |
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文献和实验该产品被引用文献
[IF={{ 4.406 }}] {Aschner Y et al. Protein Tyrosine Phosphatase-α Amplifies TGF-β-Dependent Pro-Fibrotic Signaling in Lung Fibroblasts. American Journal of Physiology-Lung Cellular and Molecular Physiology. 2020.} {FCM} {Mouse}
[IF={{ 3.266 }}] {Mitchell A et al. Human induced pluripotent stem cells ameliorate hyperoxia-induced lung injury in a mouse model. Am J Transl Res. 2020 Jan 15;12(1):292-307.} {FCM} {Human}
[IF={{ 3.022 }}] {Mitchell A et al. Assessment of iPSC Teratogenicity throughout Directed Differentiation toward an Alveolar-like Phenotype. Differentiation. 2019 Jan - Feb;105:45-53.} {FCM} {Human}
[IF={{ 2.57 }}] {Mitchell, Adam, et al. "Production of high purity alveolar-like cells from iPSCs through depletion of uncommitted cells after AFE induction." Differentiation(2017).} {FCM} {="Human"}
[IF={{ 1.38 }}] {Vadasz, Stephanie, et al. "Second and third trimester amniotic fluid mesenchymal stem cells can repopulate a de-cellularized lung scaffold and express lung markers." Journal of Pediatric Surgery (2014).} {Other} {="Human"}
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SFTPC Rabbit pAb, PE conjugated(bs-10067R-PE)-100ul
￥2980
