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- bs-1691R-HRP
- 2025年09月30日
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100ul
| 产品编号 | bs-1691R-HRP |
| 英文名称 | HCN4 Rabbit pAb, HRP conjugated |
| 中文名称 | HRP标记的环化核苷酸调控阳离子通道蛋白亚型4 |
| 英文别名 | HCN 4; Hyperpolarization activated cyclic nucleotide gated potassium channel 4; Potassium/sodium hyperpolarization activated cyclic nucleotide gated channel 4; Hyperpolarization activated Cyclic Nucleotide-gated channel 4; HCN4_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human HCN4 |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cardiovascular > Heart > Cardiac arrhythmias Neuroscience > Neurotransmission > Receptors / Channels > Ligand-Gated Ion Channels > More Channels Neuroscience > Neurotransmission > Receptors / Channels > More Ion Channels Neuroscience > Sensory System > Taste system |
| 亚基 | The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming subunits. |
| 亚细胞定位 | Membrane; Multi-pass membrane protein. |
| 组织特异性 | Highly expressed in thalamus, testis and in heart, both in ventricle and atrium. Detected at much lower levels in amygdala, substantia nigra, cerebellum and hippocampus. |
| 相似性 | Belongs to the potassium channel HCN family. Contains 1 cyclic nucleotide-binding domain. |
| 功能 | Hyperpolarization-activated ion channel with very slow activation and inactivation exhibiting weak selectivity for potassium over sodium ions. May contribute to the native pacemaker currents in heart (If) and in neurons (Ih). Activated by cAMP. May mediate responses to sour stimuli. |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | HCN4 is a member of the family of hyperpolarization activated and cyclic nucleotide gated (HCN) channels. HCN currents have been linked to pacemaker activity in the heart and brain, resting potential control, as well as neuronal plasticity. It has been shown that HCN4 channels function as receptors for sour taste, and are associated with pacemaker potential generation in the sinoatrial node. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
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文献和实验affinity chromatography and conjugated with horseradish peroxidase (HRP). Formulation Supplied as a 0.2 μm filtered solution in phosphate-buffered saline (PBS) (pH 7.0) with stabilizers. Storage Neat samples can be stored at 2° - 8° C
Western Immunoblotting of Proteins
. For a mouse monoclonal antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes
Western Immunoblotting of Proteins
antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes each with either water
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