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- Bioss
- bs-0581R-HRP
- 2025年10月15日
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100ul
| 产品编号 | bs-0581R-HRP |
| 英文名称 | IL-4 Rabbit pAb, HRP conjugated |
| 中文名称 | HRP标记的白介素4抗体 |
| 英文别名 | Interleukin-4; IL4; IL 4; B cell growth factor 1; B cell IgG differentiation factor; B Cell Stimulatory Factor 1; BCGF 1; BCGF1; Binetrakin; BSF 1; BSF1; HCGF; Hodgkin’s Cell Growth Factor; IA Inducing Factor; IGG1 induction factor; Il4e12; Interleukin 4; Interleukin 4, isoform 1; Interleukin4; Lymphocyte stimulatory factor 1; Macrophage fusion factor; Mast cell growth factor 2; MCGF2; MFF; MGC79402; Pitrakinra; T cell growth factor 2; TCGF2; IL4_MOUSE; B-cell stimulatory factor 1; BSF-1. |
| 产品应用 | WB=1:1000-5000 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from mouse IL-4 |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Tumor immunology > Cytokines > Interleukins Immunology > Adaptive Immunity > Regulatory T Cells Immunology > Innate Immunity > Cytokines > Interleukins |
| 亚细胞定位 | Secreted. |
| 相似性 | Belongs to the IL-4/IL-13 family. |
| 功能 | Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:1000-5000} |
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文献和实验affinity chromatography and conjugated with horseradish peroxidase (HRP). Formulation Supplied as a 0.2 μm filtered solution in phosphate-buffered saline (PBS) (pH 7.0) with stabilizers. Storage Neat samples can be stored at 2° - 8° C
Western Immunoblotting of Proteins
. For a mouse monoclonal antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes
Western Immunoblotting of Proteins
antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes each with either water
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