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100ul
| 产品编号 | bs-0560R-HRP |
| 英文名称 | IL13 Rabbit pAb, HRP conjugated |
| 中文名称 | HRP标记的白介素13抗体 |
| 英文别名 | Interleukin-13; Interleukin 13; Interleukin13; ALRH; BHR 1; BHR1; IL 13; IL-13; MGC116786; MGC116788; MGC116789; NC30; P600; Interleukin-13; T-cell activation protein P600; IL13_MOUSE; IL13_HUMAN. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from mouse IL-13 |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Tumor immunology > Cytokines > Interleukins Cardiovascular > Angiogenesis > Endothelial Cell Markers Immunology > Innate Immunity > Cytokines > Interleukins Kits/ Lysates/ Other > Kits > ELISA Kits > ELISA Kits > Cytokines and cytokine receptors ELISA kits |
| 亚细胞定位 | Secreted. |
| 相似性 | Belongs to the IL-4/IL-13 family. |
| 功能 | Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
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文献和实验affinity chromatography and conjugated with horseradish peroxidase (HRP). Formulation Supplied as a 0.2 μm filtered solution in phosphate-buffered saline (PBS) (pH 7.0) with stabilizers. Storage Neat samples can be stored at 2° - 8° C
Western Immunoblotting of Proteins
. For a mouse monoclonal antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes
Western Immunoblotting of Proteins
antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes each with either water
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