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- bs-10057R-HRP
- 2025年12月17日
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100ul
| 产品编号 | bs-10057R-HRP |
| 英文名称 | PCV2 capsid protein Rabbit pAb, HRP conjugated |
| 中文名称 | HRP标记的猪圆环病毒Ⅱ型衣壳蛋白抗体 |
| 英文别名 | ORF1; ORF2(PCV-2; PCV 2; Porcine circovirus2; Porcine circovirus-2.) |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from PCV2 capsid protein |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚基 | Homomultimer. Assembles in the nucleus, presumably in an immature form, then migrates to the cytoplasm once assembled as mature virion (Probable). Interacts with Rep; this interaction relocates Rep into the nucleus. |
| 亚细胞定位 | Host nucleus. Virion (Potential). |
| 相似性 | Belongs to the circoviridae capsid protein family. |
| 功能 | Self-assembles to form the virion icosahedral capsid with a T=1 symmetry. This very small capsid (17 - 22 nm in diameter) allows the virus to be very stable in the environment and resistant to some disinfectants, including detergents. Essential for the initial attachment to heparan sulfate moities and chondroitin sulfate B of the host cell surface proteoglycans. After attachment, the virus is internalized in a clathrin-, caveolae- and dynamin-independent, actin and Rho-GTPase-mediated pathway and traffics to the nucleus. The capsid protein binds and transports the viral genome and Rep across the nuclear envelope. |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Self-assembles to form the virion icosahedral capsid with a T=1 symmetry. This very small capsid (17 - 22 nm in diameter) allows the virus to be very stable in the environment and resistant to some disinfectants, including detergents. Essential for the initial attachment to heparan sulfate moities and chondroitin sulfate B of the host cell surface proteoglycans. After attachment, the virus is internalized in a clathrin-, caveolae- and dynamin-independent, actin and Rho-GTPase-mediated pathway and traffics to the nucleus. The capsid protein binds and transports the viral genome and Rep across the nuclear envelope. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
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文献和实验Mapping Protein‐Protein Interactions with Phage‐Displayed Combinatorial Peptide Libraries
the process and analysis of affinity selecting bacteriophage M13 from libraries displaying combinatorial peptides fused to either a minor or major capsid protein. Direct affinity selection uses target protein bound to a microtiter plate followed
Detecting Low‐Affinity Extracellular Protein Interactions Using Protein Microarrays
) HRP‐conjugated anti‐flag antibody Tyramide signal amplification (TSA) kit (Life Technologies, cat. no. T30953) containing
Western Immunoblotting of Proteins
. For a mouse monoclonal antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes
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