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100ul
| 产品编号 | bs-6574R-FITC |
| 英文名称 | RRBP1 Rabbit pAb, FITC conjugated |
| 中文名称 | FITC标记的核糖体结合蛋白1抗体 |
| 英文别名 | 180 kDa ribosome receptor homolog; DKFZp586A1420; ES/130; ES/130 related protein; ES130; FLJ36146; hES; Ribosome binding protein 1; Ribosome binding protein 1 homolog 180kDa; Ribosome binding protein 1 homolog; Ribosome receptor protein; RRBP 1; RRp; RRBP1_HUMAN. |
| 产品应用 | Flow-Cyt=3ug/Test Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human RRBP1 |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Tumor biomarkers Epigenetics and Nuclear Signaling > DNA / RNA > Translation > Ribosome |
| 亚细胞定位 | Endoplasmic reticulum membrane; Single-pass type III membrane protein. |
| 功能 | Acts as a ribosome receptor and mediates interaction between the ribosome and the endoplasmic reticulum membrane (By similarity). |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | RRBP1 (Ribosome-binding protein 1) acts as a ribosome receptor and mediates interaction between the ribosome and the endoplasmic reticulum membrane. |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {3ug/Test} |
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文献和实验The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
reagent; BD Biosciences 555899) (for Protocol 4) Buffer for cell staining and cell sorting (for Protocol 4; see Step 72) Complement, reconstituted from rabbit (Cedarlane CL3331) (for Protocol 3) ESC medium (for Protocols 1, 2) Freezing
rabbit serum albumin 兔血清白蛋白 R-Sc Reed-Sternberg's cell 瑞-司氏细胞 Z/R Reinheit Zahl 光密度比值 SAV small agranular vesicle 无颗粒小泡 SBA soybean agglutinin 大豆凝集素 SC secretory component 分泌成份 SCV small clear vesicle 清亮小泡 SDS sodium
变性,需在4℃进行,同时用pH8.0缓冲液稀释抗血清,以减少蛋白浓度过高而发生共沉淀。铵盐的溶解度不随温度变化而明显改变,0℃和25℃仅差3%,而钠盐则相差5倍,因此常在低温沉淀时用铵盐,室温沉淀用钠盐。铵盐对抗体的标记反应(如FITC和biotin标记时)有一定的干扰作用。 盐析法只能部分纯化抗体,更高纯度的抗体制剂可用层析法制备。IgM五聚体相对分子质量达9.7×10,比血清中任何其他蛋白都大,用分子筛层析很容易将其纯化。IgG在PH8.0时带负电荷,能与DEAE纤维素上的阳离子结合,因此可用
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