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| 产品编号 | bs-5346P |
| 英文名称 | phospho-EIF4B (Ser540) Antibody Blocking Peptide |
| 中文名称 | 磷酸化真核翻译起始因子4B封闭多肽 |
| 英文别名 | EIF 4B; EIF-4B; Eukaryotic initiation factor 4B; IF4B_HUMAN. |
| 纯化方法 | HPLC |
| 研究领域 | Epigenetics and Nuclear Signaling > DNA / RNA > Translation > Regulation |
| 亚基 | Self-associates and interacts with EIF3 p170 subunit. |
| 翻译后修饰 | Phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex. |
| 相似性 | Contains 1 RRM (RNA recognition motif) domain. |
| 功能 | Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F. |
| 保存条件 | Shipped at 4℃. Stored at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | EIF4B is required for the binding of mRNA to ribosomes. It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F. |
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文献和实验Mapping Protein‐Protein Interactions with Phage‐Displayed Combinatorial Peptide Libraries
. Fack, F., Deroo, S., Kreis, S., and Muller, C.P. 2000. Heteroduplex mobility assay (HMA) pre‐screening: An improved strategy for the rapid identification of inserts selected from phage‐displayed peptide
Synthesis and Probing of Membrane-bound Peptide Arrays
is used, follow the manufacturer's instructions for steps 9 to 11 of Method A. Alternatively, test proteins can be labeled prior to incubation with the peptide array by chemical biotinylation and subsequently detected using AP-conjugated streptavidin
Characterization of CGRP Receptor Binding
, non‐peptide CGRP receptor antagonist radioligand, [(3)H]BIBN4096BS. Eur. J. Pharmacol. 442:187‐193. Tilakaratne, N., Christopoulos, G., Zumpe, E.T., Foord, S.M., and Sexton
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