- 询价
- Signalway Antibody
- CHW017
- 2025年09月24日
- WB, IF
- Rabbit
- Hu Ms Rt Other (Wide Rtange)
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A synthetic methylated peptide corresponding to residues surrounding Arg3 of human histone H4
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Polyclonal
- 适应物种：
Hu Ms Rt Other (Wide Rtange)
- 保质期：
12 months
- 抗原来源：
Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB, IF
- 靶点：
Uniprot：P62805
- 抗体英文名：
Histone H4R3me2aConjugated Antibody
- 规格：
50ul/100ul
计算分子量:11
配方:0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
应用详情:WB: 1:50-1:200
IF:1:50-1:200
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文献和实验PROTOCOL To 1.5 mL eppendorf tubes add: 200 µg of protein extract (see Western blot protocol for protein sample preps) q.s. to 300 µL with RIPA (with protease and phosphatase inhibitors). Primary antibody (amount determined
Chromatin immunoprecipitation: optimization, quantitative analysis and data normalization
chromatin on ChIP efficiency (the recovery of DNA relative to the amount of input) for two different antibodies. With an antibody against hyperacetylated H4 (Upstate #06946), the ChIP efficiency is constant over a broad range of chromatin concentrations
from the antibody-bound and antibody-unbound fractions (from Chromatin Immunoprecipitation on Unfixed Chromatin from Cells and Tissues to Analyze Histone Modifications). Control genomic DNA should be used as well. For each PCR reaction, we use 20-50 ng of template
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