- 询价
- Signalway Antibody
- 29075
- 2025年09月23日
- WB,IHC,IF
- Rabbit
- Human;Mouse;Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A synthetic peptide of human LC3B (NP_073729.1).
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Polyclonal
- 适应物种：
Human;Mouse;Rat
- 保质期：
12 months
- 抗原来源：
Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB,IHC,IF
- 靶点：
Uniprot：Q9GZQ8
- 抗体英文名：
LC3B Polyclonal Antibody
- 规格：
50ul/100ul
计算分子量:14kDa
配方:Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 173% glycerol.
应用详情:WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
图片:
Immunofluorescence analysis of LC3B knockout 293T cells treated by chloroquine and wild-type 293T cells ??wild-type 293T cells treated by chloroquine using LC3B Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.
,
Immunohistochemistry of paraffin-embedded mouse brain using LC3B antibody at dilution of 1:100 .
,
Immunohistochemistry of paraffin-embedded rat brain using LC3B antibody at dilution of 1:100 .
,
Western blot analysis of extracts of 293T cells, using LC3B antibody at 1:1000 dilution.293T cells were treated by Chloroquine at 37?? for 20 hours.
,
Western blot analysis of extracts of various cell lines, using LC3B antibody at 1:1000 dilution.
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文献和实验Polyclonal Antibody Production
, if desired this is best accomplished by Protein A Affinity Chromatography. 上一篇：make an Antibody against a Peptides 下一篇：Immunization Protocol for Monoclonal Antibody Production
Polyclonal Antibody Production
Polyclonal Antibody Production Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation. Rabbit Immunization Initial Preparation Your antigen
Construction of Polyclonal Antibody Libraries Using Phage Display
Polyclonal antibody libraries (PCALs) are standardized mixtures of antibodies (Abs) specific for an antigen (Ag) or multi-Ag target (a poly-Ag). As the immunoglobulin (Ig) genes are cloned, the mixtures can be perpetuated, amplified
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