- 询价
- Signalway Antibody
- 28001
- 2025年09月23日
- WB IF
- Rabbit
- Human;Mouse;Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein of human c-Fos
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Polyclonal
- 适应物种：
Human;Mouse;Rat
- 保质期：
12 months
- 抗原来源：
Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB IF
- 靶点：
Uniprot：P01100
- 抗体英文名：
c-Fos Polyclonal Antibody
- 规格：
50ul/100ul
应用详情:
WB:1:500 - 1:2000
IF:1:50 - 1:200
图片:
Western blot analysis of extracts of various cell lines, using c-Fos antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
,
Immunofluorescence analysis of NIH/3T3 cells using c-Fos antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
,
Immunofluorescence analysis of C6 cells using c-Fos antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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文献和实验Polyclonal Antibody Production
, if desired this is best accomplished by Protein A Affinity Chromatography. 上一篇：make an Antibody against a Peptides 下一篇：Immunization Protocol for Monoclonal Antibody Production
Polyclonal Antibody Production
Polyclonal Antibody Production Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation. Rabbit Immunization Initial Preparation Your antigen
Construction of Polyclonal Antibody Libraries Using Phage Display
Polyclonal antibody libraries (PCALs) are standardized mixtures of antibodies (Abs) specific for an antigen (Ag) or multi-Ag target (a poly-Ag). As the immunoglobulin (Ig) genes are cloned, the mixtures can be perpetuated, amplified
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