Dnmt1 Conjugated Antibody[C493

Western Blot Analysis of Epitoped-tagged Proteins Using The Chemifluorescent Detection Method - for alkaline phosphatase conjugated antibodies

0.1% Tween-20 and 0.2% I-Block), incubate 30 min, room temperature, gently rocking. Wash three times in 20 ml 1X Western buffer (containing 0.1% Tween-20 and 0.2% I-Block) for 5 min each. Add appropriate dilution of secondary antibody conjugated

Mapping Protein Distributions on Polytene Chromosomes by Immunostaining

serum, obtained from the same species as the secondary antibody. Depending on the method employed, use anti-rabbit IgG (Fc) horseradish peroxidase (HRP) conjugate (1:100 dilution), a biotin-conjugated secondary antibody, or a fluorescence-conjugated

Quantitative immunoprecipitation of GFP-fusion proteins using the GFP-Trap

1+2, bromo adjacent homology domains 1+2. (B) Immunoblots after co-immunoprecipitations of GFP-Dnmt1 domains and Cherry-TS (C310-629 ) with the GFP-Nanotrap. G indicates GFP and C Cherry, numerics in subscript denote the first and last amino acids of Dnmt1