- 询价
- Signalway Antibody
- L35041
- 2025年09月23日
- E, WB, IH, IC
- Goat
- Rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Rabbit IgG
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Polyclonal
- 适应物种：
Rabbit
- 保质期：
12 months
- 抗原来源：
Goat
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Goat
- 应用范围：
E, WB, IH, IC
- 抗体英文名：
Goat Anti-Rabbit IgG (H&L) Biotin
- 规格：
50ul/100ul
应用详情:E (1/5000 - 1/20000), WB (1/5000 - 1/20000), IH (1/500 - 1/1000), IC (1/500 - 1/1000)
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文献和实验E L I S A w i t h p l a t e l e t s
for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS
Anti-GFP/BrdU In Situ Immunostaining
by adding 1 mL PBS, 3' each. Add 0.3 mL rabbit anti-GFP (1:200 v/v, plus 1% goat serum in PBS) and incubate 1 hr. at room temp. Wash 3x in PBS. Post-fix in 1 mL 4% paraformaldehyde x15', room temp. Wash 3x in PBS. Denature DNA with 1 mL
and wash the blot 3 times, 5-7 minutes each, with 0.5% BSA in TBST.11. Incubate with Goat anti-Rabbit/alkaline phosphatase (from Promega; 1:7500 inTBST with 5% BSA) for 1 hour at room temp with shaking. I have also used successfully Amersham ECL detection
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