- 询价
- Signalway Antibody
- 37988
- 2025年09月22日
- WB IF IP
- Mouse
- Human;Mouse;Rat
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Monoclonal
- 适应物种：
Human;Mouse;Rat
- 保质期：
12 months
- 抗原来源：
Mouse
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Mouse
- 应用范围：
WB IF IP
- 靶点：
Uniprot：P68431
- 抗体英文名：
Histone H3 Mouse Monoclonal Antibody
- 规格：
50ul/100ul
配方:Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
应用详情:Western blotting: 1:2000~1:5000
Immunofluorescence: 1:100-500
Immunoprecipitation: 1:200
图片:
Western blot analysis of 1) Hela, 2) Raw, 3) Mouse Brain Tissue, 4) Rat Brain Tissue, using #37988 diluted at 1:5,000.
,
IF analysis of Hela with DAPI (Left) and #37988(Right) diluted at 1:200.
,
1?￠Input: Hela Cell Lysate
2?￠IP product: IP dilute 1:200
Western blot analysis: primary antibody : #37988 1:2,000
Secondary antibody: Goat anti-Mouse IgG(H+L) 1:10,000.
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Detection of Histone H3 Phosphorylation in Cultured Cells and Tissue Sections by Immunostaining
. The protocol described here allows the detection of phosphorylated histones in tissue-cultured cells and tissue sections by fluorescent or bright-field immunostaining analysis. Here we used a serine 10 specific P-histone H3 antibody to determine
FACS-Based Detection of Phosphorylated Histone H3 for the Quantitation of Mitotic Cells
scanner (FACS) is described, based on the presence of an intranuclear antigen present only in mitotic cells, detected using a specific, commercially available antibody. Cell staining and FACS analysis can be done in a single day, making this a rapid
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