- 询价
- Signalway Antibody
- C56956
- 2025年09月22日
- WB, IF
- Rabbit
- Human Mouse Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A synthesized peptide derived from human Histone H2B (formyl K120)
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Monoclonal
- 适应物种：
Human Mouse Rat
- 保质期：
12 months
- 抗原来源：
Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB, IF
- 靶点：
Uniprot：Q16778
- 抗体英文名：
Histone H2B (formyl K120) Conjugated Antibody
- 规格：
50ul/100ul
计算分子量:14kDa
应用详情:WB: 1:50-1:200
IF:1:50-1:200
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文献和实验in 100µl of elution buffer at 65°C for 5 minutes;7.The eluted complexes can then be electrophoresed in denaturing PAGE and subjected to western blot analysis with an anti-Flag antibody for detection of the particular flag-tagged protein of choice (Figure
Chromatin Immunoprecipitation Protocol for Histone Modification
to keep the lysate on ice during this time. Take 50µl of lysate and use this to check the efficiency of the sonication. Proteinase K and RNaseA (500µg/ml of each) treat the sample at 37°C for 30 minutes, then transfer to 65°C for a minimum of 3 hours. Purify
," made up of fragments of up to seven nucleosomes in length, is incubated with an antiserum directed against the histone modification of interest. The antibody-bound fraction is separated from the unbound fraction and, after extraction of genomic DNA from the bound
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