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- 应用范围:
产品信息以Bioss网站为准
- 规格:
100ul/1ml
| 规格: | 100ul | 产品价格: | ¥240.0 |
|---|---|---|---|
| 规格: | 1ml | 产品价格: | ¥1580.0 |
| 产品编号 | bs-0293M-FITC |
| 英文名称 | Mouse Anti-Rat IgG H&L, FITC conjugated |
| 中文名称 | FITC标记的小鼠抗大鼠IgG H&L |
| 英文别名 | Mouse Anti-Rat IgG H&L (FITC); Immunoglobulin G; |
| 产品应用 | IF=1:100-1000, Flow-Cyt=1:100-1000, ICC/IF=1:100-1000 Not yet tested in other applications. |
| 交叉反应 | Rat |
| 抗体来源 | Mouse |
| 免疫原 | Native Rat IgG |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Polyclonal |
| 浓度 | 2.0 mg/ml |
| 储存液 | 10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol. |
| 保存条件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Immunoglobulin G(IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. |
| 应用 | 推荐稀释比例 |
| {IF} | {1:100-1000} |
| {Flow-Cyt} | {1:100-1000} |
| {ICC/IF} | {1:100-1000} |
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文献和实验[IF={{ 6.6 }}] {Ze-Nan Huang. et al. Melatonin alleviates oxidative stress-induced injury to nucleus pulposus-derived mesenchymal stem cells through activating PI3K/Akt pathway. J ORTHOP TRANSL. 2023 Nov;43:66} {IF} {Rat}
[IF={{ 2.9 }}] {Jieru Zhang. et al.Dexmedetomidine activates mitophagy and protects against pyroptosis in oxygen-glucose deprivation/reperfusion-induced brain damage via PINK1/Parkin pathway activation.JOURNAL OF BIOENERGETICS AND BIOMEMBRANES.2025 Feb 22.} {IF} {Rat}
[IF={{ 2.5 }}] {Ji-Yang Tan. et al. Porous hydrogel loaded with BMSCs overexpressing IL-4 promotes tendon-bone healing by activating the TGF-β/Smad2/3 pathway. NEW J CHEM. 2025 Jul;:} {IF} {Rat}
E L I S A w i t h p l a t e l e t s
for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS
Immunohistochemistry using Anti-Ganglioside Antibodies
')2 or FITC-conjugated goat anti-mouse IgG F (ab')2 diluted at 1:1000 each with 1% BSA in PBS for 1 hr. at room temperature. 7. Wash with 1% BSA in PBS 5 times. 8. Apply drops of fluorescence mounting medium on section and mount micro cover glass
Immunofluorescent Staining of Mouse and Rat Leukocytes
(or 50 µl wash buffer for negative controls). Mix by gently vortexing or tapping. Incubate at 4°C for 20-40 min in the dark. Wash 2X with 200 µl wash buffer (or 3X if a biotin-conjugated primary antibody is used). After each centrifugation, 350 x g
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