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- 应用范围:
产品信息以Bioss网站为准
- 规格:
100ul/1ml
| 规格: | 100ul | 产品价格: | ¥240.0 |
|---|---|---|---|
| 规格: | 1ml | 产品价格: | ¥1580.0 |
| 产品编号 | bs-0358D-HRP |
| 英文名称 | Donkey Anti-Guinea Pig IgG H&L, HRP conjugated |
| 中文名称 | 辣根过氧化物酶标记的驴抗豚鼠IgG H&L |
| 英文别名 | Donkey Anti-Guinea Pig IgG H&L (HRP); Immunoglobulin G; |
| 产品应用 | WB=1:1000-10000, IHC-P=1:100-500, IHC-F=1:100-1000, ELISA=1:1000-10000 Not yet tested in other applications. |
| 交叉反应 | Guinea Pig |
| 抗体来源 | Donkey |
| 免疫原 | Native Guinea Pig IgG |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Polyclonal |
| 浓度 | 2.0 mg/ml |
| 储存液 | 10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol. |
| 保存条件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:1000-10000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-1000} |
| {ELISA} | {1:1000-10000} |
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文献和实验[IF={{ 9.207 }}] {Chen, Xingxing. et al. Differential expression and roles of Huntingtin and Huntingtin-associated protein 1 in the mouse and primate brains. CELL MOL LIFE SCI. 2022 Nov;79(11):1-20} {IP,WB} {Mouse, Monkey, Human}
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for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS
我是用来做筛选IgG型单克隆抗体,用它做二抗,筛到的阳性克隆化和扩大培养后用SIGMA亚类试剂盒鉴定却是IgM,郁闷!!!这个试剂盒能与IGM交叉?是不是抗轻链?我这种方式不对吗?谁知道有较好的抗鼠IgG而与IgM不交叉的抗体?? 试剂盒说明书:Anti-mouse IgG-HRP Antibody FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. R&D Systems, Inc. 1-800-343-7475 2/21/06
-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)
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