- ￥400 - 800
- Proteintech
- CL647-98087
- 2025年10月09日
- FC (Intra)
- Rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
CoraLite® Plus 647 Fluorescent Dye
- 适应物种：
human
- 宿主：
Rabbit
- 应用范围：
FC (Intra)
- 靶点：
IL-6
- 抗体英文名：
CoraLite® Plus 647 Anti-Human IL-6 Rabbit Recombinant Antibody
- 抗体名：
CoraLite® Plus 647 Anti-Human IL-6 Rabbit Recombinant Antibody
- 规格：
25tests/100tests
| 规格： | 25tests | 产品价格： | ￥400.0 |
|---|---|---|---|
| 规格： | 100tests | 产品价格： | ￥800.0 |
经过测试的应用
| Positive FC (Intra) detected in | LPS and Brefeldin A treated human PBMCs |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 5 ul per 10^6 cells in 100 μl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-98087 targets IL-6 in FC (Intra) applications and shows reactivity with human samples.
| 经测试应用 | FC (Intra) |
| 经测试反应性 | human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | interleukin 6 (interferon, beta 2) |
| 别名 | IL6, 240670D12, B-cell stimulatory factor 2, IFN-beta-2, IL 6 |
| 计算分子量 | 212 aa, 24 kDa |
| GenBank蛋白编号 | BC015511 |
| 基因名称 | IL6 |
| Gene ID (NCBI) | 3569 |
| ENSEMBL Gene ID | ENSG00000136244 |
| 偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
| 最大激发/发射波长 | 654 nm / 674 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P05231 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.
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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
4 Antibody 22. Rabbit Anti-SeV Antibody 23. Alexa Fluor® 488 Goat Anti-Mouse IgG (H L) Antibody 24. Alexa Fluor® 594 Goat Anti-Mouse IgG (H L) Antibody 25. Alexa Fluor® 488 Goat Anti-Rabbit IgG (H L) Antibody 26. Alexa Fluor® 594
protein depends upon the species that the antibody was raised in. Protein A binds well to rabbit, cat, human, pig and guinea pig IgG as well as mouse IgG2a and IgG2b. Protein G binds strongly to IgG from cow, goat, sheep, cow, horse, rabbit and guinea pig
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