相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG1
- 保存条件:
Store at -80°C.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
human
- 宿主:
Mouse
- 应用范围:
ELISA, Non-Neutralization
- 靶点:
NeutraControl IL-6
- 抗体英文名:
NeutraControl IL-6 Monoclonal antibody, PBS Only
- 抗体名:
NeutraControl IL-6 Monoclonal antibody, PBS Only
- 规格:
100ug
产品信息
69501-1-PBS targets IL-6 in ELISA, Non-Neutralization applications and shows reactivity with human samples.
| 经测试应用 | ELISA, Non-Neutralization |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Product name: HumanKine® recombinant human IL-6 protein Source: -derived, Tag: Domain: of Sequence: |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | interleukin 6 (interferon, beta 2) |
| 别名 | IL6, IL-6, 1E9B8, B-cell stimulatory factor 2, IFN-beta-2 |
| 基因名称 | IL6 |
| Gene ID (NCBI) | 3569 |
| ENSEMBL Gene ID | ENSG00000136244 |
| 偶联类型 | Unconjugated |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P05231 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存 | Store at -80°C. |
背景介绍
Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.
This antibody is a neutralizing control antibody for IL-6, the immunogen is the same as Neutrakine 69001-1-Ig but could not neutralize human IL-6.
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验1. 杂交瘤阳性克隆的筛选与克隆化 在HAT培养基中生长的杂交瘤细胞,只有少数是分泌预定特异性单克隆抗体的细胞,因此,必须进行筛选和克隆化。通常采用有限稀释法进行杂交瘤细胞的克隆化培养。采用灵敏、快速、特异的免疫学方法,筛选出能产生所需单克隆抗体的阳性杂交瘤细胞,并进行克隆扩增。经过全面鉴定其所分泌单克隆抗体的免疫球蛋白类型、亚类、特异性、亲和力、识别抗原的表位及其分子量后,及时进行冻存。2. 单克隆抗体的大量制备 单克隆抗体的大量制备重要采用动物体内诱生法和体外培养法。
猴白细胞介素 - 6( IL- 6) 酶联免疫 分析( ELISA ) 试剂 盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定猴血清,血浆及相关液体样本中 白细胞介素-6(IL-6) 含量。 实验原理 : 本试剂盒应用 双抗体 夹心法测定 标本 中 猴白细胞介素 - 6( IL- 6) 水平。用纯化的 猴白细胞介素 - 6( IL- 6)抗体 包被微孔板,制成固相 抗 体,往包被 单抗 的微孔中依次
过夜。(5)用少许PBS将交联物全部洗出,收集洗出液,加1/20V体积新鲜配制的5mg/ml NaBH4溶液,混匀,室温作用30分钟;再加入3/20V NaBH4溶液,混匀,室温作用1小时(或4℃过夜)。(6)将交联物过Sephadex G200或Sepharose 6B(2.6×50cm)层析纯化,分管收集第一峰。(7)酶结合物质量鉴定:克分子比值测定酶量(mg/ml)=OD403×0.4IgG量(mg/ml)=(OD280-OD403×0.3)×0.62克分子比值(E/P)=酶量×4/IgG量
技术资料暂无技术资料 索取技术资料











