- ￥2800
- Proteintech
- CL750-80017
- 2025年10月09日
- WB
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human calreticulin protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
CoraLite® Plus 750 Fluorescent Dye
- 适应物种：
human, mouse, rat
- 宿主：
Rabbit
- 应用范围：
WB
- 靶点：
calreticulin
- 抗体英文名：
CoraLite® Plus 750-conjugated calreticulin Recombinant monoclonal antibody
- 抗体名：
CoraLite® Plus 750-conjugated calreticulin Recombinant monoclonal antibody
- 规格：
100ul
经过测试的应用
| Positive WB detected in | Jurkat cells, K-562 cells |
| Positive FC (Intra) detected in | K-562 cells, Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-80017 targets calreticulin in WB applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag26064 Product name: Recombinant human calreticulin protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 248-417 aa of BC002500 Sequence: KKPEDWDEEMDGEWEPPVIQNPEYKGEWKPRQIDNPDYKGTWIHPEIDNPEYSPDPSIYAYDNFGVLGLDLWQVKSGTIFDNFLITNDEAYAEEFGNETWGVTKAAEKQMKDKQDEEQRLKEEEEDKKRKEEEEAEDKEDDEDKDEDEEDEEDKEEDEEEDVPGQAKDEL |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | calreticulin |
| 别名 | CALR, Calregulin, 2B17 |
| 计算分子量 | 60 kDa |
| 观测分子量 | 50-55 kDa |
| GenBank蛋白编号 | BC002500 |
| 基因名称 | Calreticulin |
| Gene ID (NCBI) | 811 |
| RRID | AB_3673721 |
| 偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
| 最大激发/发射波长 | 755 nm / 780 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P27797 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CALR, also named as grp60, ERp60, HACBP, CRP55, CRTC and Calregulin, belongs to the calreticulin family. It is a molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. CALR is a ER marker. It interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. CALR interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. The MW of CALR migrates aberrantly at 55 kDa by SDS-PAGE. Some study provided that it's a new possibility for CRT-mediated tumor immune prevention and treatment.
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文献和实验Screening Colonies by Hybridization with Radiolabeled Probe
dH2 O) Denaturing solution (0.5 N NaOH, 1.5 M NaCl) (dissolve 43.83 g NaCl in 300 ml dH2 O, add 50 ml 10 N NaOH stock solution , dH2 O ad 500 ml) Neutralizing solution (1.5 M NaCl, 0.5 M Tris ・ Cl [pH 7.4]) (100 ml dH
体内后，通过糖苷键结合参与代谢，转换为葡萄糖醛酸轭合物或硫酸轭合物。所以β-受体激动剂的检测通常需要酶解步骤，β-葡萄糖醛酸酶是一种可以使葡萄糖醛酸苷键加水分解的酶，它可以把葡萄糖醛酸轭合物或硫酸轭合物的糖苷键切断，释放出游离态。目前，β-受体激动剂类药物残留的检测方法包括高效液相色谱法（HPLC）、气相色谱-质谱法（GC-MS）、液相色谱-质谱/质谱法（LC-MS/MS）、毛细管电泳电气化学检测法。下面介绍使用新型重组酶 IMCSzyme® β-葡萄糖醛酸酶与 MicroSep® DPX 枪头式分散固相
体 对于包涵体的纯化，包涵体的前处理是很重要的。 包涵体中主要含有重组蛋白,但也含有一些细菌成分,如一些外膜蛋白、质粒DNA和其它杂质。洗涤常用1%以下的中性去垢剂，如Tween、Triton、Lubel和 NP40等加EDTA和还原剂2-巯基苏糖醇（DTT）、β-巯基乙醇等反复多次进行，因去垢剂洗涤能力随溶液离子强度升高而加强，在洗涤包涵体时可加50 mM NaCL。这样提取的包涵体纯度至少可达50%以上，而且可保持元结构。也可用低浓度的盐酸胍或尿素/中性去垢剂/EDTA/还原剂等洗去
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