- ￥1500 - 3280
- Proteintech
- 82349-1-RR
- 2025年10月09日
- WB, IHC, FC (Intra), ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 宿主：
Rabbit
- 应用范围：
WB, IHC, FC (Intra), ELISA
- 靶点：
Phospho-IkB Alpha (Ser32/36)
- 抗体英文名：
Phospho-IkB Alpha (Ser32/36) Recombinant monoclonal antibody
- 抗体名：
Phospho-IkB Alpha (Ser32/36) Recombinant monoclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1500.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2480.0 |
| 规格： | 150ul | 产品价格： | ￥3280.0 |
经过测试的应用
| Positive WB detected in | Calyculin A treated HeLa cells, Calyculin A treated HSC-T6 cells, Calyculin A treated NIH/3T3 cells, rat liver tissue, mouse liver tissue |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | Calyculin A treated HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
82349-1-RR targets Phospho-IkB Alpha (Ser32/36) in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, FC (Intra), ELISA |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig, zebrafish |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha |
| 别名 | IκBα (Ser 32/36), p IkB Alpha, p IkB Alpha (Ser32/36), p IkB Alpha Ser32/36, p IkB α |
| 计算分子量 | 36 kDa |
| 观测分子量 | 36 kDa |
| GenBank蛋白编号 | BC004983 |
| 基因名称 | IkB Alpha |
| Gene ID (NCBI) | 4792 |
| RRID | AB_3073626 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P25963 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime. The antibody also detects the phosphorylated form of the NFKBIA (IkB Alpha) (PMID: 16904979, PMID: 28990531 PMID: 10455908).
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse | WB | Adv Sci (Weinh) Reprogramming Lung Redox Homeostasis by NIR Driven Ultra-Small Pd Loaded Covalent Organic Framework Inhibits NF-κB Pathway for Acute Lung Injury ImmunotherapyAuthors - Doudou Lei |
| mouse | WB | EMBO Mol Med Targeting HPK1 inhibits neutrophil responses to mitigate post-stroke lung and cerebral injuriesAuthors - Tingting Zhang |
| mouse,zebrafish | WB | Sci Total Environ Glycidol induces necroptosis and inflammation through autophagy-necrosome pathway in renal cell and miceAuthors - Jing Lu |
| mouse | WB | Int J Biol Macromol Structural characterization of an apple polysaccharide and its anti-inflammatory effect through suppressing TLR4/NF-κB signalingAuthors - Shanbo Ma |
| mouse | WB | Int J Biol Macromol Flammulina velutipes polysaccharide exerts immunomodulatory function involving RSAD2 to regulate the NF-κB/MAPK signaling pathway in RAW264.7 macrophage cells and in mouse spleen cellsAuthors - Qiuyi Wang |
| human,mouse | WB | Int J Biol Macromol In vitro and in vivo immunomodulatory activity of acetylated polysaccharides from Cyclocarya paliurus leavesAuthors - Yang Zhang |
下调不能说明活性降低，因为决定GSK3-beta的活性的还与其磷酸化的比例和磷酸化的位点有关。所以你除了要做GSK3-beta总的蛋白表达之外，还要做磷酸化的GSK3-beta的蛋白表达。 （2）应该活性形式和非活性形式都做。一般来讲，GSK3-beta在Ser9位点磷酸化之后活性收到抑制，而在216位点磷酸化之后，其活性收到加强。因此建议将GSK3-beta的两个磷酸化位点都做了，另外还要同时检测GSK3-beta的总蛋白表达，这样才能全面的说明问题。
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
3.1 and 3.6 ). This will provide a “specific activity” measurement relative to total protein in the cell, and is themost accurate measure of total GSK3 activity, or2. Immunoblot using antibodies to phospho-Ser21 (GSK3a)or phospho-Ser9 (GSK3b) (see Sections 3.1 –
/ampoule 重组人干扰素α 2a(rHuIFN-alpha2(alpha-A)) 冻干粉 63,000IU/ampoule 第八页 重组人干扰素α 2b (rHuIFN-alpha2(alpha-2b)) 冻干粉 70,000IU/ampoule 重组人干扰素β ser - 17 (rHuIFN-beta (ser-17)) 冻干粉 6,000IU/ampoule 人成纤维细胞干扰素β(HuIFN-beta) 冻干粉 4,000IU/ampoule 重组人干扰素γ冻干粉 标准品 80,000
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