- ￥920 - 2290
- Proteintech
- AB-65246
- 2025年10月09日
- FC
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG2b
- 保存条件：
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
Atlantic Blue™ Fluorescent Dye
- 适应物种：
Human
- 宿主：
Mouse
- 应用范围：
FC
- 靶点：
CD14
- 抗体英文名：
Atlantic Blue™ Anti-Human CD14 (26IC)
- 抗体名：
Atlantic Blue™ Anti-Human CD14 (26IC)
- 规格：
25tests/100tests
| 规格： | 25tests | 产品价格： | ￥920.0 |
|---|---|---|---|
| 规格： | 100tests | 产品价格： | ￥2290.0 |
经过测试的应用
| Positive FC detected in | human PBMCs |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in 100 μl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
AB-65246 targets CD14 in FC applications and shows reactivity with Human samples.
| 经测试应用 | FC |
| 经测试反应性 | Human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | CD14 molecule |
| 别名 | CD14, CD14 molecule |
| 计算分子量 | 375 aa, 40 kDa |
| GenBank蛋白编号 | BC010507 |
| 基因名称 | CD14 |
| Gene ID (NCBI) | 929 |
| ENSEMBL Gene ID | ENSG00000170458 |
| RRID | AB_2935209 |
| 偶联类型 | Atlantic Blue™ Fluorescent Dye |
| 最大激发/发射波长 | 404 nm / 458 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P08571 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.
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文献和实验Immunostaining with Anti-Digoxigenin Alkaline Phosphatase
Materials Anti-digoxigenin-AP conjugate (Roche #11093274910, 150U/200 uL, $168) Levamisole (Sigma #T1512, 2 g, $10) (blocks endogenous phosphatase activity) BCIP (Roche #11383221001, 3 mL, $49) NBT (Roche #11383213001, 3 mL, $48)
Protocol for anti-HA antibody Western Blotting
SJF 4/00 1) Run gel (in 1:10 running/transfer buffer(10x) and H2O for a total of 1litre) at 150 volts until leading bromophenol blue band is nearing the bottom edge of the glass plates or according to the expected migration of the protein
Protocol for anti-HA antibody Western Blotting
1) Run gel (in 1:10 running/transfer buffer(10x) and H2O for a total of 1litre) at 150 volts until leading bromophenol blue band is nearing the bottom edge of the glass plates or according to the expected migration of the protein
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