- ￥3200
- Proteintech
- CL594-18989
- 2025年10月09日
- FC (Intra)
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
CoraLite®594 Fluorescent Dye
- 适应物种：
human, mouse, rat
- 宿主：
Rabbit
- 应用范围：
FC (Intra)
- 靶点：
GLI2-Specific
- 抗体英文名：
CoraLite®594-conjugated GLI2-Specific Polyclonal antibody
- 抗体名：
CoraLite®594-conjugated GLI2-Specific Polyclonal antibody
- 规格：
100ul
经过测试的应用
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-18989 targets GLI2-Specific in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | FC (Intra) |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | GLI family zinc finger 2 |
| 别名 | Gli2, Thp, Tax helper protein, GLI Kruppel family member GLI2, Gli 2 |
| 计算分子量 | 168 kDa |
| 观测分子量 | 167 kDa， 88 kDa |
| GenBank蛋白编号 | NM_005270 |
| 基因名称 | GLI2 |
| Gene ID (NCBI) | 2736 |
| RRID | AB_2919850 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q0VGT2 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GLI2, also named as THP, belongs to the GLI C2H2-type zinc-finger protein family. GLI2 play a role during embryogenesis. It binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1. It is implicated in the transduction of SHH signal. Mutation of GLI2 will cause of holoprosencephaly type 9 (HPE9). Four possible isoforms (hGli2a, b, g, and d) are formed by combinations of two independent alternative splicings, and all the isoforms could bind to a DNA motif, TRE2S. hGli2 a, b, g, and d. These isoforms encode 133-, 131-, 88-, and 86-kDa proteins, respectively.
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文献和实验Immunofluorescence or IF is a technique allowing the visualization of a specific protein or antigen in cells or tissues by binding a specific antibody chemically conjugated with a fluorescence dye. Immunofluorescent staining is widely used
溶液(STZ 浓度从2×10-4 至2×10-2 )，然后制备标准曲线。 文献：Specific polyclonal-based immunoassays for sulfathiazole. Nuria Pastor-Navarro, Cristina García-Bover, Ángel Maquieira and Rosa Puchades. Analytical and Bioanalytical Chemistry. Volume 379, Numbers
Sequential RNA and DNA fluorescence in situ hybridization
Back to top Figures An example of sequential RNA and DNA FISH. RNA FISH was performed against IL-4 mRNA in mouse T helper type 2 cells and visualized with Alexa594-conjugated tyramide using the TSA technique (left). After RNase treatment, DNA FISH
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