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- 详细信息
- 技术资料
- 免疫原:
Recombinant human KAT2A protein
- 亚型:
IgG
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Recombinant
- 标记物:
Unconjugated
- 适应物种:
human, rat
- 宿主:
Rabbit
- 应用范围:
WB, IHC, IF/ICC, FC (Intra), ELISA
- 靶点:
KAT2A/GCN5
- 抗体英文名:
KAT2A/GCN5 Recombinant monoclonal antibody
- 抗体名:
KAT2A/GCN5 Recombinant monoclonal antibody
- 规格:
50ul/100ul/150ul
| 规格: | 50ul | 产品价格: | ¥1500.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2480.0 |
| 规格: | 150ul | 产品价格: | ¥3280.0 |
经过测试的应用
| Positive WB detected in | HEK-293 cells, A431 cells, HeLa cells, MCF-7 cells, LNCaP cells, HSC-T6 cells |
| Positive IHC detected in | human colon cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
| Positive FC (Intra) detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84669-2-RR targets KAT2A/GCN5 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), ELISA |
| 经测试反应性 | human, rat |
| 免疫原 |
CatNo: Ag29005 Product name: Recombinant human KAT2A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 366-449 aa of BC032743 Sequence: EIYGANSPIWESGFTMPPSEGTQLVPRPASVSAAVVPSTPIFSPSMGGGSNSSLSLDSAGAEPMPGEKRTLPENLTLEDAKRLR |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | K(lysine) acetyltransferase 2A |
| 别名 | 242113C7, EC:2.3.1.-, EC:2.3.1.48, GCN5, GCN5L2 |
| 计算分子量 | 94 kDa |
| 观测分子量 | 94 kDa |
| GenBank蛋白编号 | BC032743 |
| 基因名称 | KAT2A |
| Gene ID (NCBI) | 2648 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q92830 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GCN5, encoded by KAT2A, is a member of nuclear A-type histone acetyltransferase (HAT) which has direct impact on gene transcription. Additionally, GCN5 has roles in cell cycle regulation, DNA replication, and DNA repair, highlighting it as key player in the maintenance of genome stability. GCN5 is a coactivator of c-MYC oncogene protein and has oncogenic roles in various cancers.
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