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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human INHA protein
- 亚型:
IgG
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
human, mouse, rat
- 宿主:
Rabbit
- 应用范围:
WB, IHC, ELISA
- 靶点:
Inhibin alpha
- 抗体英文名:
Inhibin alpha Polyclonal antibody
- 抗体名:
Inhibin alpha Polyclonal antibody
- 规格:
50ul/100ul/150ul
| 规格: | 50ul | 产品价格: | ¥1350.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2200.0 |
| 规格: | 150ul | 产品价格: | ¥3000.0 |
经过测试的应用
| Positive WB detected in | mouse ovary tissue, mouse testis tissue, rat ovary tissue |
| Positive IHC detected in | mouse ovary tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27331-1-AP targets Inhibin alpha in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA |
| 文献引用应用 | IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag25999 Product name: Recombinant human INHA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 233-366 aa of BC006391 Sequence: STPLMSWPWSPSALRLLQRPPEEPAAHANCHRVALNISFQELGWERWIVYPPSFIFHYCHGGCGLHIPPNLSLPVPGAPPTPAQPYSLLPGAQPCCAALPGTMRPLHVRTTSDGGYSFKYETVPNLLTQHCACI |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | inhibin, alpha |
| 别名 | INHA, Inhibin A, Inhibin α, Inhibin-A, Inhibinα |
| 计算分子量 | 40 kDa |
| 观测分子量 | 40 kDa |
| GenBank蛋白编号 | BC006391 |
| 基因名称 | Inhibin alpha |
| Gene ID (NCBI) | 3623 |
| RRID | AB_3085946 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P05111 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Inhibin, a heterodimeric member of the TGF family, consists of an alpha (α) subunit (coded by INHA) and a beta (β) subunit, which combine to produce either inhibin A (αβA) or inhibin B (αβB). Inhibin complexes negatively regulate follicle stimulating hormone secretion from the pituitary gland. Inhibins have also been implicated in regulating numerous cellular processes including cell proliferation, apoptosis, immune response and hormone secretion.
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse | IF | Apoptosis CCDC134 enhances ovarian reserve function and angiogenesis by directly interacting with INHA in a mouse model of premature ovarian insufficiencyAuthors - Xiangrong Cui |
accumulation by LPS, as TLR4 can use either MyD88 or TRIF. The net result of these interactions was activation of the transcription factors NF-{kappa}B and AP-1, and synthesis of TNF-{alpha} mRNA. TNF-{alpha} acts through an apparent autocrine pathway
:FITC 羊抗小鼠(绿色)稀释度 1:700 室温下孵育 1 小时。复染色:Hoechst(蓝色)细胞核染色,1:4,000 室温下孵育 1 小时, 细胞定位:路易体内含物;放大倍数:20x。 注射了 1 型小鼠α-突触核蛋白 PFFs(SPR-324)的大鼠脑免疫组织化学分析。实验物种:Sprague-Dawley 大鼠(母),在大鼠脑中有两个注射点,分别是 AP+1.6、ML+2.4、DV-4.2 和 AP-1.4、ML+0.2、DV-2.8,每个注射点注射 2 µL 1 型小鼠 α-突触核蛋白
)、Anti-IgA (alpha)】 在一些特殊的实验里,只需要检测样本里的IgG或者IgM,常规的Anti-IgG (H+L)由于与IgG的重链和轻链都有结合反应,而IgG、IgM、IgA都有保守的轻链结构域,因此不能特异性的检测某种类型的IgG或者IgM。这时候就需要针对特殊类型Ig分子的二抗。***捷备有全面的二抗现货产品并能提供最完整的二抗产品线。主要二抗品牌为全球最大的二抗产品Jackson二抗以及前沿抗体生产商EarthOx。按照标记分,有HRP、AP、生物素等标记二抗,FITC
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