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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human S100B protein
- 亚型:
IgG
- 保存条件:
Store at -80°C.
- 克隆性:
Recombinant
- 标记物:
Unconjugated
- 适应物种:
human
- 宿主:
Rabbit
- 应用范围:
Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
- 靶点:
S100B
- 抗体英文名:
S100B Recombinant monoclonal antibody, PBS Only (Detector)
- 抗体名:
S100B Recombinant monoclonal antibody, PBS Only (Detector)
- 规格:
100ug
产品信息
82271-11-PBS targets S100B as part of a matched antibody pair:
MP01165-2: 82271-10-PBS capture and 82271-11-PBS detection (validated in Cytometric bead array, Sandwich ELISA)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag7440 Product name: Recombinant human S100B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-92 aa of BC001766 Sequence: MSELEKAMVALIDVFHQYSGREGDKHKLKKSELKELINNELSHFLEEIKEQEVVDKVMETLDNDGDGECDFQEFMAFVAMVTTACHEFFEHE |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | S100 calcium binding protein B |
| 别名 | S100 beta, Protein S100 B, Protein S100-B, S100 calcium-binding protein B, S-100 protein beta chain |
| 计算分子量 | 11 kDa |
| GenBank蛋白编号 | BC001766 |
| 基因名称 | S100 Beta |
| Gene ID (NCBI) | 6285 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P04271 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
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文献和实验-HCl缓冲液作为平衡液和洗脱液,流速每分钟1ml。第一个蛋白峰即为脱盐的抗体溶液。透析法是将透析袋于2% NaHCO3,1mmol/L EDTA溶液中煮10分钟,用蒸馏水清洗透析袋内外表面,再用蒸馏水煮透析袋10分钟,冷至室温即可使用(并可于0.2mol/L EDTA溶液中,4℃保存备用)。将盐析样品装入透析袋中,对50-100倍体积的PBS或Tris-HCl缓冲液透析(4℃)12-24小时,其间更换5次透析液,用萘氏试剂(碘化汞11.5g,碘化钾8g,加蒸馏水50ml,待溶解后,再加20% NaOH
,0.2mM EDTA的PH磷酸盐缓冲液(PBS)溶解,于50-100倍体积的上述PBS中4℃透析过夜. (11) 取少量透析后样品适当稀释后,以紫外分光光度计检测蛋白含量,SDS-PAGE检测抗体 纯度.
法小量抽提质粒 (1)将细菌沉淀[上述步骤(3)所得]重悬于100μl预冷的溶液Ⅰ(50mmol/L葡萄糖,25mmol/l Tris –HCl pH8.0, 10mmol/L EDTA)中,剧烈振荡。 (2)加200μl新配制的溶液Ⅱ(0.2mol/l NaOH, 1%SDS),缓和振荡,水浴5min。 (3)加150μl预冷溶液Ⅲ(50mol/L KAC 60ml, 冰乙酸11.5ml,水28.5ml),缓和振荡,冰浴5min。 (4)4℃
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