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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG2a
- 保存条件:
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性:
Recombinant
- 标记物:
CoraLite® Plus 488 Fluorescent Dye
- 适应物种:
human
- 宿主:
Mouse
- 应用范围:
FC
- 靶点:
ICAM-1/CD54
- 抗体英文名:
CoraLite® Plus 488 Anti-Human ICAM-1/CD54 (15.2) Mouse IgG2a Recombinant Antibody
- 抗体名:
CoraLite® Plus 488 Anti-Human ICAM-1/CD54 (15.2) Mouse IgG2a Recombinant Antibody
- 规格:
25tests/100tests
| 规格: | 25tests | 产品价格: | ¥400.0 |
|---|---|---|---|
| 规格: | 100tests | 产品价格: | ¥800.0 |
经过测试的应用
| Positive FC detected in | human PBMCs |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in 100 μl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-65567 targets ICAM-1/CD54 in FC applications and shows reactivity with human samples.
| 经测试应用 | FC |
| 经测试反应性 | human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | intercellular adhesion molecule 1 |
| 别名 | CD54, ICAM-1, ICAM1, ICAM 1, BB2 |
| 计算分子量 | 90 kDa |
| GenBank蛋白编号 | BC015969 |
| 基因名称 | ICAM-1 |
| Gene ID (NCBI) | 3383 |
| ENSEMBL Gene ID | ENSG00000090339 |
| RRID | AB_3672882 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P05362 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).
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文献和实验Culturing BG01V Human Embryonic Stem Cells with Mouse Embryonic Fibroblast (MEF)-Conditioned Media
(approximately 1.0 x 106 cells/60 mm plate) on the Cultrex BME coated plate in Conditioned Media containing 4 ng/mL of recombinant human FGF basic. Change the media daily. Monitor the cells for the desired confluency. Figure
Procedure for Culturing BG01V Human Embryonic Stem Cells with Human Feeder Cell Conditioned Medium
Recombinant human FGF basic (R&D Systems, Catalog # 233-FB , 4114-TC , or equivalent) Accutase (Innovative Cell Technologies, Catalog # AT104 or equivalent) Cultrex® Basement Membrane Extract (BME) (R&D Systems, Catalog # 3433-005-01 or equivalent
-20 in 50 mM Na-phosphate buffer, pH 7.3. Goat-anti-mouse antibodies labeled with AlexaFluor-488 were from Invitrogen, Inc. (dye/protein ratio 7). Microscope glass slides, 3 × 1 in., and 1 mm thick, were from VWR. Water was purified by Milli-Q
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