- ￥2800
- Proteintech
- PE-82674-10
- 2025年10月09日
- FC (Intra), ELISA
- Rabbit
- human
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
PE Fluorescent Dye
- 适应物种：
human
- 宿主：
Rabbit
- 应用范围：
FC (Intra), ELISA
- 靶点：
Phospho-STAT1 (Tyr701)
- 抗体英文名：
PE-conjugated Phospho-STAT1 (Tyr701) Recombinant monoclonal antibody
- 抗体名：
PE-conjugated Phospho-STAT1 (Tyr701) Recombinant monoclonal antibody
- 规格：
100ul
经过测试的应用
| Positive FC (Intra) detected in | IFN gamma treated THP-1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.03 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
PE-82674-10 targets Phospho-STAT1 (Tyr701) in FC (Intra), ELISA applications and shows reactivity with human samples.
| 经测试应用 | FC (Intra), ELISA |
| 经测试反应性 | human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | signal transducer and activator of transcription 1, 91kDa |
| 别名 | p STAT1, p STAT1 (Tyr701), p STAT1 Tyr701, Phospho STAT1, Phospho STAT1 (Tyr701) |
| 计算分子量 | 83 kDa |
| 观测分子量 | 84-91 kDa |
| GenBank蛋白编号 | BC002704 |
| 基因名称 | STAT1 |
| Gene ID (NCBI) | 6772 |
| 偶联类型 | PE Fluorescent Dye |
| 最大激发/发射波长 | 496 nm, 565 nm / 578 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P42224 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
STAT1 (signal transducers and activators of transcription 1) is a member of the STAT protein family. STAT family members are phosphorylated by the receptor associated kinases, and then form homo-or heterodimers that translocate to the cell nucleus where they act as transcription activators. The phosphorylation of STAT1 at Tyr701 promotes the dimerization of STAT1 and its translocation into the nucleus where it binds the GAS promoter to allow the transcription of inflammatory response genes(PMID: 31408005). The transcript of STAT1 undergoes alternative splicing, resulting in two isoforms: STAT1α (91 kDa) and STAT1β (84 kDa)(PMID: 20159032).
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
Figure 18.20.1 The specificity of phospho‐motif antibodies. HeLa cells were serum‐starved for 24 hr prior to being stimulated with 50 ng/ml IGF‐1 or 100 nM PMA for 10 min. Lysates were immunoblotted with anti‐Akt/PKB phospho‐motif (left panel
步：选择抗体 在弹出的界面中列出了所有已选抗原可选的荧光素种类，并且在每一个通道内仅可选择一个抗原。 点击其中一种抗原与荧光素搭配，即可查看可供选择的所有货号信息，这里以抗CD56抗体偶联PE为例，在跳出的窗口中可以对比可选货号的信息，这里建议选择克隆号以REA开头的REAfinityTM重组抗体，比传统的单克隆抗体有更高的特异性和可重复性，点击select即可将选择保存至配色方案中。 依次在每个通道中选择需要的抗体即可完成配色。再次点击右上方的Continue，即可得到一份可以下载保存
染色法1-6、如前所述，用经稀释的未标记的单克隆抗体对细胞样品处理；7、 将细胞沉淀重新悬浮于50μl的HAB中，混匀，于室温中静置1分钟以上；8、 加入50μl经荧光标记的第二抗体稀释液；9、 轻轻振荡后于4℃下暗置20分钟；10、 用1ml的缓冲液洗两次，于250g下离心5分钟；11、 将样品重新悬浮于1ml缓冲液中，于4℃下避光保存待用。注意：此法并不适用于Ig表面染色或用抗体直接对FC受体进行染色。
技术资料暂无技术资料 索取技术资料
