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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 保存条件:
Store at -80°C.
- 克隆性:
Recombinant
- 标记物:
Unconjugated
- 适应物种:
rat
- 宿主:
Rabbit
- 应用范围:
Sandwich ELISA, Indirect ELISA
- 靶点:
PAI-1/Serpin E1
- 抗体英文名:
Rat PAI-1/Serpin E1 Recombinant monoclonal antibody, PBS Only (Detector)
- 抗体名:
Rat PAI-1/Serpin E1 Recombinant monoclonal antibody, PBS Only (Detector)
- 规格:
100ug
产品信息
86765-1-PBS targets PAI-1/Serpin E1 as part of a matched antibody pair:
MP02642-1: 86765-2-PBS capture and 86765-1-PBS detection (validated in Sandwich ELISA)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | Sandwich ELISA, Indirect ELISA |
| 经测试反应性 | rat |
| 免疫原 |
CatNo: Eg3256 Product name: Recombinant Rat Serpin E1/PAI-1 protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 24-402 aa of NM_012620.1 Sequence: SPLPESHTAQQATNFGVKVFQHVVQASKDRNVVFSPYGVSSVLAMLQLTTAGKTRQQIQDAMGFNISERGTAPALRKLSKELMGSWNKNEISTADAIFVQRDLELVQGFMPHFFKLFRTTVKQVDFSEVERARFIINDWVERHTKGMISDLLAKGAVNELTRLVLVNALYFNGQWKTPFLEASTHQRLFHKSDGSTISVPMMAQNNKFNYTEFTTPDGHEYDILELPYHGETLSMFIAAPFEKDVPLSAITNILDAELIRQWKSNMTRLPRLLILPKFSLETEVDLRGPLEKLGMTDIFSSTQADFTSLSDQEQLSVAQALQKVKIEVNESGTVASSSTAILVSARMAPTEMVLDRSFLFVVRHNPTETILFMGQLMEP |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | serine (or cysteine) peptidase inhibitor, clade E, member 1 |
| 别名 | Serpin E1/PAI-1, Endothelial plasminogen activator inhibitor, PAI, Pai1, PAI-1 |
| 计算分子量 | 45 kDa |
| GenBank蛋白编号 | NM_012620.1 |
| 基因名称 | Serpine1 |
| Gene ID (NCBI) | 24617 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P20961 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
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文献和实验至 1 升。室温保存。 2. 甲醛溶液(无甲醇的)。 3. 孵育缓冲液:将 0.5 g 牛血清白蛋白(bovine serum albumin,BSA)溶解在 100 ml 的 1 X PBS 中。保存在 4°C。 B. 固定 1. 离心收集细胞,弃上清。 2. 将细胞重悬在 0.5-1 ml PBS 中。加入甲醛溶液至终浓度为 2-4%。 3. 37°C 固定 10 分钟。 4. 放在冰上降温 1 分钟。 5. 做细胞外染色不需要细胞膜打孔时,继续 D1
efficiency was 20-30%. (B ) The normalised percentage of apoptosis is shown for the various constructs. The apoptotic index was normalised using the function (% apopotosis in X / % apoptosis in eGFP-C1/E1A) × 100. The apoptotic index was normalised
Typical modification sites: RGG box or RXR sequence motifs R-arginine, G-glycine, X-any aminoacid. Enzymes catalysing protein arginine methylation: PRMT1 from rat and the human homologue HRMT1L2, human PRMT2 (HRMT1L1), rat and human PRMT
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