- ￥1500 - 3280
- Proteintech
- 80046-3-RR
- 2025年10月09日
- WB, IF/ICC, ELISA
- Rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
human
- 宿主：
Rabbit
- 应用范围：
WB, IF/ICC, ELISA
- 靶点：
Phospho-HSP27 (Ser78)
- 抗体英文名：
Phospho-HSP27 (Ser78) Recombinant monoclonal antibody
- 抗体名：
Phospho-HSP27 (Ser78) Recombinant monoclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1500.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2480.0 |
| 规格： | 150ul | 产品价格： | ￥3280.0 |
经过测试的应用
| Positive WB detected in | Calyculin A treated PC-3 cells |
| Positive IF/ICC detected in | Calyculin A treated HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
80046-3-RR targets Phospho-HSP27 (Ser78) in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA |
| 经测试反应性 | human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | heat shock 27kDa protein 1 |
| 别名 | Hsp27 (phospho S78), HSP27 (Ser78), HSP27-Ser78, Phospho HSP27 (Ser78), Phospho-HSP27 |
| 计算分子量 | 19-23 kDa |
| 观测分子量 | 25-30 kDa |
| GenBank蛋白编号 | BC012768 |
| 基因名称 | HSPB1 |
| Gene ID (NCBI) | 3315 |
| RRID | AB_3670462 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P04792 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HSPB1, also named as HSP27, belongs to the mammalian small HSP family widely expressed in cells including the cytoplasm and nucleus. It has been reported that HSPB1 plays a key role in stress resistance, actin organization and numerous biological processes. HSPB1 can be phosphorylated in different stimulation such as cytokines, growth factors and peptide hormones on the site of Ser15, Ser78 and Ser82 by MAPKAP2 kinase. The phosphorylation of HSPB1 leads to a decrease in the size of HSPB1 oligomers. HSPB1 is associated with amyotrophic lateral sclerosis (ALS) (PMID: 8542580, 11960785, 10383393, 32231288)
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Using Phospho‐Motif Antibodies to Determine Kinase Substrates
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