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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 保存条件:
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性:
Recombinant
- 标记物:
CoraLite® Plus 647 Fluorescent Dye
- 适应物种:
human
- 宿主:
Rabbit
- 应用范围:
FC
- 靶点:
NKp46/NCR1
- 抗体英文名:
CoraLite® Plus 647 Anti-Human NKp46/NCR1 Rabbit Recombinant Antibody
- 抗体名:
CoraLite® Plus 647 Anti-Human NKp46/NCR1 Rabbit Recombinant Antibody
- 规格:
25tests/100tests
| 规格: | 25tests | 产品价格: | ¥400.0 |
|---|---|---|---|
| 规格: | 100tests | 产品价格: | ¥800.0 |
经过测试的应用
| Positive FC detected in | human PBMCs |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in a 100 µl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-98340 targets NKp46/NCR1 in FC applications and shows reactivity with human samples.
| 经测试应用 | FC |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Eg1364 Product name: Recombinant Human NKp46/NCR1 protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 22-258 aa of / Sequence: QQQTLPKPFIWAEPHFMVPKEKQVTICCQGNYGAVEYQLHFEGSLFAVDRPKPPERINKVKFYIPDMNSRMAGQYSCIYRVGELWSEPSNLLDLVVTEMYDTPTLSVHPGPEVISGEKVTFYCRLDTATSMFLLLKEGRSSHVQRGYGKVQAEFPLGPVTTAHRGTYRCFGSYNNHAWSFPSEPVKLLVTGDIENTSLAPEDPTFPADTWGTYLLTTETGLQKDHALWDHTAQNLLR |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | natural cytotoxicity triggering receptor 1 |
| 别名 | 242549D12, CD335, hNKp46, LY94, Natural killer cell p46-related protein |
| 计算分子量 | 34kDa |
| 基因名称 | NCR1 |
| Gene ID (NCBI) | 9437 |
| 偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
| 最大激发/发射波长 | 654 nm / 674 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O76036-1 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
NKp46, also known as NCR1 or CD335, is an Ig-like superfamily cell surface receptor that is highly conserved in mammals (PMID: 22021440). NKp46 is a type I transmembrane glycoprotein consisting of two extracellular Ig-like domains, a transmembrane domain, and an intracellular tail (PMID: 9730896). It is expressed on NK cells, rare T-cell subsets and a mucosal population of NKp46+ innate lymphoid cells (PMID: 9730896; 22021440). NKp46 is the major triggering receptor involved in the natural cytotoxicity (PMID: 10359120; 10092106; 15356098).
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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
Immune Response Biomarker Profiling
a total of 5 wash steps. 8) During the wash steps, mix 2.5 L Alexa Fluor® 647 goat anti-human IgG antibody with 5 mL Washing Buffer per array to obtain a final antibody concentration of 1 g/mL. Store on ice until use. Optional: add Alexa Fluor®®
')2 Fragment(Alexa Fluor® 555 Conjugate)#4413 Anti-Rabbit IgG(H+L), F(ab')2 Fragment(Alexa Fluor® 647 Conjugate)#4414 抗小鼠: Anti-Mouse IgG(H+L), F(ab')2 Fragment(Alexa Fluor® 488 Conjugate)#4408 Anti-Mouse IgG(H+L), F(ab')2 Fragment(Alexa Fluor
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