- ￥600 - 1200
- Proteintech
- CL405-98349
- 2025年10月09日
- FC
- Mouse
- rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG2a
- 保存条件：
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
CoraLite® Plus 405 Fluorescent Dye
- 适应物种：
rabbit
- 宿主：
Mouse
- 应用范围：
FC
- 靶点：
CD3
- 抗体英文名：
CoraLite® Plus 405 Anti-Rabbit CD3 Mouse Recombinant Antibody
- 抗体名：
CoraLite® Plus 405 Anti-Rabbit CD3 Mouse Recombinant Antibody
- 规格：
25ug/100ug
| 规格： | 25ug | 产品价格： | ￥600.0 |
|---|---|---|---|
| 规格： | 100ug | 产品价格： | ￥1200.0 |
经过测试的应用
| Positive FC detected in | rabbit splenocytes |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL405-98349 targets CD3 in FC applications and shows reactivity with rabbit samples.
| 经测试应用 | FC |
| 经测试反应性 | rabbit |
| 免疫原 |
CatNo: Eg3525 Product name: recombinant rabbit CD3E+CD3G共表达 protein Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 10007197 of Sequence: / |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | T-cell surface glycoprotein CD3 epsilon chain |
| 别名 | CD3 epsilon + CD3 gamma, CD3 epsilon/CD3 gamma, CD3 epsilon, CD3 gamma, CD3e |
| 基因名称 | CD3E |
| Gene ID (NCBI) | 100008616 |
| 偶联类型 | CoraLite® Plus 405 Fluorescent Dye |
| 最大激发/发射波长 | 399 nm / 422 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD3 epsilon(CD3ε) and CD3 gamma (CD3γ) are transmembrane subunits of the CD3 complex, which is essential for the assembly and surface expression of the T cell receptor (TCR). All of the CD3 subunits carry the so-called immunoreceptor tyrosine-based activation motif (ITAM) in the intracytoplasmic region. The signals are initiated by the specific phosphorylation of two tyrosine residues located in the conserved ITAM (PMID: 21269565).
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文献和实验7211011 小鼠淋巴细胞分离液 达优 100339 Ultra-LEAF™ Purified anti-mouse CD3ε Antibody BioLegend 102115 Ultra-LEAF™ Purified anti-mouse CD28 Antibody BioLegend CD3/CD28 Streptamer® 多聚体法： 货号 名称
Th17 Polarization of Mouse CD4 Cells
实验试剂 Sterile PBS Cell culture medium Sterile plastic petridishes RBC Lysis Buffer [Details: Biolengend, cat.#420301] Anti-mouse CD3ε, clone145-2C11[Details: LEAF™format, cat.#100314]
ANALYSIS OF PROTEINS BY IMMUNOPRECIPITATION
although other techniques can be used. The choice of immobilized antibody binding protein depends upon the species that the antibody was raised in. Protein A binds well to rabbit, cat, human, pig and guinea pig IgG as well as mouse IgG2a and IgG2b . Protein G binds
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