- ￥5800
- Proteintech
- 84369-1-PBS
- 2025年10月09日
- Cytometric bead array, Indirect ELISA
- Rabbit
- rat
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -80°C.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
rat
- 宿主：
Rabbit
- 应用范围：
Cytometric bead array, Indirect ELISA
- 靶点：
CD44
- 抗体英文名：
Rat CD44 Recombinant monoclonal antibody, PBS Only (Capture/Detector)
- 抗体名：
Rat CD44 Recombinant monoclonal antibody, PBS Only (Capture/Detector)
- 规格：
100ug
产品信息
84369-1-PBS targets CD44 as part of a matched antibody pair:
MP01236-1: 84369-2-PBS capture and 84369-1-PBS detection (validated in Cytometric bead array)
MP01236-2: 84369-3-PBS capture and 84369-1-PBS detection (validated in Cytometric bead array)
MP01236-3: 84369-1-PBS capture and 84369-4-PBS detection (validated in Cytometric bead array)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | Cytometric bead array, Indirect ELISA |
| 经测试反应性 | rat |
| 免疫原 |
CatNo: Eg1458 Product name: Recombinant Rat CD44 protein (rFc Tag) (HPLC verified) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 22-271 aa of XM_006234631.3 Sequence: QQQIDLNITCRYAGVFHVEKNGRYSISRTEAADLCEAFNTTLPTMAQMELALSKGFETCRYGFIEGHVVIPRIHPNAICAANNTGVYILLASNTSHYDTYCFNASAPLEEDCTSVTDLPNSFDGPVTITIVNRDGTRYSKKGEYRTHQEDIDASNIIDEDVSSGSTIEKSTPEGYILHTDLPTSQPTGDRDDAFFIGSTLATSDGDSSMDPRGGFDTVTHGSELAGHSSGNQDSGVTTTSGPARRPQIPE |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | Cd44 molecule |
| 别名 | CD44 antigen, ECMR-III, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, Hermes antigen |
| 计算分子量 | 40 kDa |
| GenBank蛋白编号 | XM_006234631.3 |
| 基因名称 | Cd44 |
| Gene ID (NCBI) | 25406 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O70509 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
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文献和实验2次；加入RNA酶10ug和碘化丙啶（PI）0.3ml重悬细胞（4°C，30分钟）。用流式细胞仪以FL2红色荧光条件检测。应用Modfit LT 2.0 软件分析细胞周期。 取第三代细胞用流式细胞仪作细胞周期及细胞免疫表型（CD29/CD44）的鉴定。 3. 细胞表面分子免疫标记的鉴定 a. 方法：直接免疫荧光法 b. 试剂及材料：单克隆抗体 CD29 PE，CD44 PE,及同型对照抗体；细胞洗液：含2%BSA、0.1%NaN3的PBS；固定
至 1 升。室温保存。 2. 甲醛溶液（无甲醇的）。 3. 孵育缓冲液：将 0.5 g 牛血清白蛋白（bovine serum albumin，BSA）溶解在 100 ml 的 1 X PBS 中。保存在 4°C。 B. 固定 1. 离心收集细胞，弃上清。 2. 将细胞重悬在 0.5-1 ml PBS 中。加入甲醛溶液至终浓度为 2-4%。 3. 37°C 固定 10 分钟。 4. 放在冰上降温 1 分钟。 5. 做细胞外染色不需要细胞膜打孔时，继续 D1
Direct tissue explant method for derivation and study of extravillous trophoblast
well culture plates (Costar) Pastettes 9 cm petri dishes 5 cm petri dishes Rat tail collagen type I (Becton Dickenson, distributed by Stratech) Matrigel (Becton Dickenson, distributed by Stratech) 10 x DMEM (Sigma
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